Objective To observe the rule of formation, growth and figuration of femur in the process from embryonic development to maturation, and analyse the formation of bone growth center, volume of bone layer and variation of bone shape. Methods CT scan technology was employed to obtain the imaging information of femur development process of rabbits, and the CT imaging information was processed with Mimics Medical Image Processing software. Time-phase and stratified space analysis of rule of femur figuration and variation of volume of bone layer were performed with unit bone volume method. Results Femur has multiple bone growth centers. The femur bone first emerged as spongy bone during embryonic process, and gradually got harden and formed the compact bone and enamel bone along with the growth. Finally, the femur bone developed its optimal shape and bone mass distribution. Conclusion The femur is developed by combination of 4 independent bone growth centers. There is obvious change in volume of bone layer and position in the process of bone growth, while stabilized state is eventually arrived.