Original article (Basic research)

Influence of short hairpin RNA of EMMPRIN on invasion and migration of SGC-7901 cells

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  • Department of Gastroenterology, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China

Online published: 2013-02-06

Abstract

Objective To construct the short hairpin RNA (shRNA) of extracellular matrix metalloproteinase inducer (EMMPRIN), and investigate its influence on invasion and migration of human gastric cancer SGC-7901 cells. Methods Four groups of human EMMPRIN siRNA were designed and synthesized, double-stranded shRNA was annealed, which was then inserted into pGenesil-1 plasmid. The interference plasmid of EMMPRIN with significant silencing effect was screened by sequencing, RT-PCR and Western blotting, and was transfected into SGC-7901 cells by Lipofectamine 2000. Transwell assay was employed to determine the effect of recombinant interference plasmid on invasion and migration of SGC-7901 cells. Results The interference plasmid pshRNA-EMMPRIN was constructed and screened. After transfection, the expression of EMMPRIN mRNA and protein in SGC-7901 cells significantly decreased. Transwell assay revealed that the number of cells with invasion and migration significantly decreased in SGC-7901 cells after transfection with recombinant interference plasmid. Conclusion The downregulation of EMMPRIN expression can significantly decrease the invasion and migration of tumor cells, which lays a foundation for the further study of gene therapy of tumor with EMMPRIN.

Cite this article

KANG Yu-jun, WANG Chuan, JIANG Zheng, et al . Influence of short hairpin RNA of EMMPRIN on invasion and migration of SGC-7901 cells[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2013 , 33(1) : 12 . DOI: 10.3969/j.issn.1674-8115.2013.01.003

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