Objective To investigate the influence of transfer medium EmbryoGlue on pregnancy outcome of in vitro fertilization-embryo transfer (IVF-ET). Methods Patients undergoing IVF-ET were enrolled, and were divided into EmbryoGlue group (EmbryoGlue as transfer medium, 232 cycles) and G-2 group (G-2 as transfer medium, 268 cycles). The clinical parameters and pregnancy outcomes were compared between groups. Results There was no significant difference in the age, duration of infertility, number of unsuccessful cycles, thickness of endometrium, number of good quality embryo, mean number of embryo transfer between groups (P>0.05). The clinical pregnancy rate, implantation rate, multiple pregnancy rate in EmbryoGlue group were significantly higher than those in G-2 group (P<0.05). The prevalence of ectopic pregnancy in EmbryoGlue group was significantly lower than that in G-2 group (P<0.05). Conclusion As an embryo transfer medium, EmbryoGlue can improve the pregnancy rate and implantation rate of IVF-ET.