Objective To investigate the attitudes and influencing factors of obtaining information about human immunodeficiency virus/acquired immune deficiency syndrome(HIV/AIDS) via internet among female sex workers(FSWs) and men who have sex with men (MSM). Methods FSWs and MSM selected from Xinjiang, Sichuan, Guangxi and Chongqing were surveyed with questionnaires, which included demographics, behavior characteristics, ways to obtain information about HIV/AIDS and the desirable ways to obtain it. Results Internet was the first choice for more than 80% of MSM to obtain information about HIV/AIDS. For FSWs, 27.54% obtained information about HIV/AIDS via internet, and 31.66% were willing to obtain information via internet. Logistic regression analysis revealed that ethnics, marital status, age, education background, workplace and frequency of sex service per week were influencing factors of acceptability to obtain information about HIV/AIDS via internet among FSWs, and ethnics, age, education background and seeking sex partner through internet were influencing factors of acceptability among MSM. Conclusion Obtaining information about HIV/AIDS via internet is acceptable for MSM, and it is feasible and useful to conduct health education via internet for MSM. While viable methods should be adopted to conduct health education for FSWs in order to achieve better effects.