Objective To analyze the spatial distribution and influencing factors of tuberculosis in Chongqing using spatial autocorrelation analysis and spatial autoregressive model. Methods The data on tuberculosis of 40 districts (counties) in Chongqing from December 2008 December to 2011 were collected, and spatial autocorrelation analysis and spatial regressive analysis were performed using GeoDa_0.9.5.i software. Results The global Moran´sⅠ values of tuberculosis in Chongqing from 2008 to 2011 were 0.310, 0.399, 0.327, and 0.306, respectively, which indicated that there was a spatial positive correlation among each year in the incidences of tuberculosis in Chongqing. Spatial regressive analysis revealed that the incidence of tuberculosis in Chongqing was associated with the unemployment rate of urban area (Z=2.150, P=0.032). Conclusion The spatial clusterings of tuberculosis are found in Chongqing from 2008 to 2011, and the incidence of tuberculosis in Chongqing is associated with the unemployment rate of urban area at the spatial level.