Objective To investigate the correlation between mean platelet volume (MPV) and myocardial bridging (MB). Methods The data of MPV of patients with isolated MB confirmed by coronary angiography (MB group, n=326) and those with normal coronary angiographic findings (control group, n=334) were retrospectively analysed. All the study objectives were stratified by age and gender, and the differences in MPV were compared between subgroups. The risk factors of MB were explored by multivariate Logistic regression analysis. Results There was no significant difference in MPV between MB group and control group [(8.88±1.27) fl vs (8.78±1.27) fl, P>0.05], and there was also no significant difference in MPV between the same gender of two groups (P>0.05). The cutoff point of age was determined as 44 years with favorable sensitivity and specificity. In those aged no higher than 44 years, MPV in MB group was significantly higher than that in control group [(9.49±1.30) fl vs (8.62±1.31) fl, P<0.01], while there was no significant difference in MPV between two groups in the other age subgroups (P>0.05). In those aged no higher than 44 years, multivariate regression analysis revealed that MB was significantly related to MPV (OR=1.71, 95%CI 1.16-2.51, P<0.01), while was not related to gender, blood pressure and blood lipid (P>0.05). Conclusion MB may be associated with the increase of MPV, and the relevance may be more significant in young patients with MB.