上海交通大学学报(医学版) ›› 2017, Vol. 37 ›› Issue (10): 1342-.doi: 10.3969/j.issn.1674-8115.2017.10.006

• 论著(基础研究) • 上一篇    下一篇

半胱氨酸组织蛋白酶S基因敲除对高岭土诱导小鼠交通性脑积水的影响

刘枫荻 1*,赵 蓉 1*,史妍慧 1,李格飞 1,吴依兰 1,庄美婷 1,尹家文 1,杜小霞 2,周佳君 3#,刘建仁 1#   

  1. 1. 上海交通大学 医学院附属第九人民医院神经内科,上海 200011;2. 华东师范大学物理系上海磁共振重点实验室,上海 200062;3. 浙江中医 药大学附属杭州市西溪医院神经内科,杭州 311100
  • 出版日期:2017-10-28 发布日期:2017-11-01
  • 通讯作者: 周佳君,电子信箱:13588267956@163.com。刘建仁,电子信箱:liujr021_201410@126.com。# 共同通信作者
  • 作者简介:刘枫荻,女(1991—),硕士生;电子信箱:liufengdi199119@sina.com。赵蓉,女(1987—),住院医师,博士;电子信箱:zr1703@126.com。* 共同 第一作者
  • 基金资助:
     上海市科学技术委员会创新行动计划基础研究重点项目(14JC1404300);上海交通大学医学院多中心临床研究项目(DLY201614);上海市科学技 术委员会生物医药支撑项目(16411953100);上海交通大学医学院—中国科学院神经科学研究所脑疾病临床研究中心2015 年率先启动项目(2015NKX006);上 海申康医院发展中心临床科技创新项目(SHDC12015310)

Influence of cathepsin S deficiency on development of communicating hydrocephalus in mice induced by kaolin#br#

LIU Feng-di1*, ZHAO Rong1*, SHI Yan-hui1, LI Ge-fei1, WU Yi-lan1, ZHUANG Mei-ting1, YIN Jia-wen1, DU Xiao-xia2, ZHOU Jia-jun3#, LIU Jian-ren1#   

  1. 1. Department of Neurology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; 2. Department of Physics and Shanghai Key Laboratory of Magnetic Resonance, East China Normal University, Shanghai 200062, China; 3. Department of Neurology, Xixi Hospital of Hangzhou, Zhejiang University of Traditional Chinese Medicine, Hang zhou 311100, China
  • Online:2017-10-28 Published:2017-11-01
  • Supported by:
    Research Innovation Project from Shanghai Municipal Science and Technology Commission, 14JC1404300; Clinical Research Project from Shanghai Jiao Tong University School of Medicine, DLY201614; Biomedicine Key Program from Shanghai Municipal Science and Technology Commission, 16411953100; Project (2015) from SHSMU-ION Research Center for Brain Disorders, 2015NKX006; Clinical Science and Technology Innovation Project of Shanghai Shen Kang Hospital Development Center, SHDC12015310

摘要: 目的 · 观察半胱氨酸组织蛋白酶 S(CatS)基因敲除对高岭土诱导小鼠交通性脑积水严重程度的影响。方法 · 针对 8 只 CatS 基 因敲除(CatS -/-)小鼠和16 只野生型(WT)C57BL/6 小鼠,通过枕大池注射高岭土混悬液的方法建立交通性脑积水模型,建模前及 建模后1 周利用头颅磁共振(MRI)扫描测量小鼠侧脑室体积并计算侧脑室指数,比较2 组小鼠脑积水严重程度。结果 · 建模后1 周 CatS -/- 组死亡 1 只(12.5%), WT 组死亡 2 只(12.5%), 2 组间死亡率的差异无统计学意义(P=1.000);头颅 MRI 结果显示存活 CatS -/组和WT 组小鼠均有不同程度脑室扩大,建模前CatS -/- 组(n=8)侧脑室指数为0.05±0.01,WT 组(n=16)侧脑室指数为0.04±0.01 (P=0.720)。建模后 1周CatS -/- 组(n=7)侧脑室指数为 0.13±0.02,其中侧脑室指数增加 1倍以上者 5只,占 71.4%;WT 组(n=14)侧 脑室指数为 0.11±0.01,侧脑室指数增加 1 倍以上者 10 只,占 71.4%; 2 组比较,差异无统计学意义(P=0.950)。 结论 · 枕大池注射高岭 土混悬液后 1周,小鼠侧脑室明显扩大伴脑积水发生,模型成功率高;CatS 基因敲除对高岭土诱导小鼠脑交通性脑积水发展无明显影响。

关键词: 脑积水, 高岭土, 半胱氨酸组织蛋白酶 S, 基因敲除

Abstract:  Objective · To evaluate the influence of cathepsin S(CatS) on the severity of communicating hydrocephalus in a kaolin injected mouse model.  Methods · Kaolin suspension was injected to 8 CatS knock-out (CatS -/-) mice and 12 wild type (WT) C57BL/6 mice through cisterna magna to establish communicating hydrocephalus mouse model. Cerebral magnetic resonance imaging (MRI) was used before and 1 week after kaolin injection to compare lateral ventricular volume. Lateral ventricular index was calculated to analyze the severity of hydrocephalus.  Results · One week after kaolin injection, 1 in CatS -/- group and 2 in WT group died. The mortality rate was 12.5% each and there was no significant difference (P=1.000). MRI results showed varying degrees of ventriculomegaly in both groups. Lateral ventricular index of CatS -/-group (n=8) and WT group (n=16) before kaolin injection was 0.05±0.01 and 0.04±0.01 respectively (P=0.720). One week after kaolin injection, lateral ventricular index of CatS-/- group (n=7) and WT group (n=14) was 0.13±0.02 and 0.11±0.01 respectively (P=0.950). In each group, in 71.4% of mice, lateral ventricular index enlarged twice or more.  Conclusion · One week after kaolin injection into cisterna magna, lateral ventricles enlarges obviously, indicating hydrocephalus occurs, with high success rate. CatS gene deficiency has no significant influence on the development of communicating hydrocephalus.

Key words:  hydrocephalus, kaolin, Cathepsin S, gene knock-out