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Effects of DHA on inflammatory response in human periodontal ligament cells induced by P.gingivalis  LPS

ZHOU Zi-chao, SUN Meng-jun, SHU Rong   

  1. Department of Periodontology, Shanghai Ninth Peoples Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Research Institute of Stomatology, Shanghai Key Lab of Stomatology, Shanghai 200011, China
  • Online:2016-05-28 Published:2016-05-26
  • Supported by:

    National Nature Science Foundation of China， 81570977

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Abstract:

Objective To investigate the effects of docosahexaenoic acid (DHA) on   periodontal ligament cells (hPDLCs)-expressed inflammatory factors induced by Porphyromonas gingivalis (P.gingivalis) lipopolysaccharides (LPS). Methods HPDLCs were cultured and identified in vitro. The effects of different concentrations of DHA on the cell viability were detected with MTT assay. The concentrations with no effect on the cell viability were selected according to the results of MTT assay and the effects of DHA on hPDLCs-expressed inflammatory factors interleukin-6 (IL-6), IL-8, and IL-1β induced by P.gingivalis LPS were detected at gene and protein levels with real-time PCR and ELISA. Results In the range of 25-100 μmol/L, DHA had no effect on the cell viability in hPDLCs and down-regulated hPDLCs-expressed inflammatory factors induced by P.gingivalis LPS in a concentration-dependent manner. Conclusion In a certain range of concentrations, DHA can down-regulated hPDLCs-expressed inflammatory factors induced by P.gingivalis LPS in a concentration-dependent manner without affecting the cell viability, suggesting the possibility of applying DHA to the anti-inflammatory therapy of periodontosis.

Key words: docosahexaenoic acid, Porphyromonas gingivalis, lipopolysaccharides, human periodontal ligament cells, inflammatory response