Abstract:

Objective To analyse the genotypes and antifungal resistance of Candida glabrata isolated from Renji Hospital affiliated to Shanghai Jiaotong University School of Medicine between 2011 and 2012 in order to investigate the drug resistance and epidemic situation. Methods Genotyping was conducted by pulsed-field gel electrophoresis(PFGE), and susceptibility tests were carried out with ATB-FUNGUS system. Results Forty-nine strains of Candida glabrata were divided into 17 genotypes by PFGE, including 12 strains of type A, 11 of type B, 4 of type C, 3 of type D, type E and type F each, 2 of type G and type H each, 1 of type I, type J and type K each. The rates of susceptibility to 5-fluorocytosine, amphotericin B, voriconazole, fluconazol and itraconazole were 100%, 100%, 89.8%, 85.7% and 40.8% respectively. There was no significant difference in the rates of susceptibility to the same drug among different genotypes (P>0.05). Conclusion Type A was the major genotype of Candida glabrata in Renji Hospital. There is a high cross-resistance level in Candida glabrata to triazole drugs, which should be attached great importance.

Key words: pulsed-field gel electrophoresis, Candida glabrata, cross-resistance