›› 2018, Vol. 38 ›› Issue (6): 647-.doi: 10.3969/j.issn.1674-8115.2018.06.011

• Original article (Clinical research) • Previous Articles     Next Articles

Effect of serum IgA1 on human mesangial cells and of TGF-β1 in patients of IgA nephropathy with different renal function progress

KONG Ling-yun, JIANG Geng-ru   

  1. Department of Nephrology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Online:2018-06-28 Published:2018-07-03
  • Supported by:
    Science and Technology Commission of Shanghai Municipality, 12140903002; Projects of Shanghai Municipal Health and Family Planning Commission, 20144Y0145

Abstract: Objective · To extract serum IgA1 patients with IgA nephropathy (IgAN) (end stage renal disease vs long-term srenal function) to explore the effect on proliferation rate of human mesangial cells (HMCs) and the level of transforming growth factor-β1 (TGF-β1). Methods · Nine patients with primary IgAN were divided into rapidly progressive group (n6) and long-term sgroup (n3). Jacalin affinity chromatography and sephacryl S-200HRmolecular sieves were used to distract serum IgA1. HMCs were cultured and co-cultivated with different IgA1 concentration (10, 50, 250 and 1 000 μg/mL)at point of 12 h and 24 h respectively. The proliferation rate was measuredcell counting kit-8 (CCK8). The of TGF-β1 mRNA was measured via quantitative real-time PCR (qRT-PCR). Enzyme-linked immunosorbent assay (ELISA) was used to detect TGF-β1 protein. Results · Serum IgA1 IgAN patients with different renal functions (end stage renal disease vs long-term srenal function) activated proliferation of HMC significantly, presenting with time-dependence and concentration-dependence. The highest value showed at 250 μg/mL and 1 000 μg/mL respectively. Serum IgA1 in two groups of patients statistically increased the of TGF-β1 mRNA and protein. In group with end stage renal disease, the summit stood at 10 μg/mL and started to decreasedegrees afterwards; while in group with long-term srenal function, the level of TGF-β1 increased in a parallel manner with the serum IgA1. Conclusion · Serum IgA1 IgAN patients with different renal functions (end stage renal disease vs long-term srenal function) can both promote the proliferation of HMC. There is no dramatical difference observed with in10-50 μg/mL, but the IgA1 group with end stage renal disease reveals a stronger effect on TGF-β1, in accordance with the pathological type of the patients (IgA sclerosis), suggesting the prognostic value of serum IgA.

Key words: IgA nephropathy, renal function, human mesangial cell, transforming growth factor-&, beta, 1

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