Objective To investigate the related factors for career well-being among scientific researchers, and put forward suggestions for the construction of research team. Methods Young scientific researchers aged between 31 and 45 years were randomly selected for questionnaire survey from Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine (n=58), Shanghai Institute of Hematology (n=33) and Basic Medical College of Shanghai Jiaotong University (n=39). The questionnaire was composed of career well-being recognition and mood experience scale. The career well-being recognition dimensions included salary,

career development, supervisor, welfare, reward, working procedure, colleague, task and interpersonal coordination, and Positive and Negative Affect ScheduleRevised (PANAS-R) was employed in mood experience test. Besides, in-depth interview was carried out in two core scientific researchers in Shanghai Institute of Hematology. Results The career well-being index of young scientific researchers ranged between 105 and 120, with the average of 112.48 (124 points in total). The scores of salary, welfare and working procedure were lower. There were significant differences in the degree of satisfaction towards career development, working

procedure and supervisor among different age groups (P<0.05). The in-depth interview of researchers in Shanghai Institute of Hematology revealed that both the living pressure and working pressure were high, while there was sense of dignity and achievement in working. Conclusion More reasonable salary distribution system should be established, and individualized incentive system should be constructed in order to prevent the outflow of talents.