• Original article (Basic research) • Previous Articles     Next Articles

Prediction and analysis of the distribution of T cell epitopes encoded by the specific RD12 region of Mycobacterium tuberculosis

YE Juan1, ZHANG Shu-lin2, LIU Wen-di1   

  1. 1.Key Laboratory of Medical Immunology, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China; 2.Department of Immunology and Microbiology, Basic Medicine Faculty of Shanghai Jiao Tong University, Shanghai 200025, China
  • Online:2014-01-28 Published:2014-01-29
  • Supported by:

    Major Science and Technology Program in the National “12th 5-year Plan” of China，2013ZX10003002-005； National Natural Science Foundation of China， 81271794； Shanghai Science and Technology Commission Foundation， 12441903300

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Abstract:

Objective To predict and analyze distribution of CD4+ T cell and CD8+ T cell epitopes encoded by the specific RD12 region of Mycobacterium tuberculosis (MTB). Methods The amino acid sequences of the antigens were obtained by online NCBI database. The homology between MTB complex and human proteins was analyzed by BLAST. The distribution of CD8+ T cell epitopes was predicted by SYFPEITHI, NETCTL, BIMAS, and NetMHC databases. The distribution of CD4+ T cell epitopes was predicted by NetMHC databases. After analyzing distribution of CD4+ and CD8+ T cell epitopes, the superior epitopes were selected. Results Sixteen candidate CD8+ T cell epitopes binding to 4 amino acids and 234 strong and 505 weak candidate CD4+ T cell epitopes binding to 15 amino acids were screened preliminarily, respectively. Thirteen superior epitopes were selected. Conclusion The candidate T cell epitopes may help lay a foundation for the research of multiepitope candidate vaccines and early diagnosis of tuberculosis.

Key words: Mycobacterium tuberculosis, specific RD12 region, T cell, epitope analysis