Abstract:

Objective  To observe changes of the drug concentration of rabbit VX2 tumor model intervened by the combination of embedded ultrasound tumor hyperthermia instrument and pegylated liposomal doxorubicin (PLD). Methods  Twelve Zelanian female rabbits with VX2 tumors in superficial thigh muscle were randomly divided into the hyperthermia+PLD group (n=4), PLD group (n=4), and doxorubicin group (n=4) when the diameter of tumor was larger than 10 mm. For hyperthermia+PLD group, embedded ultrasound tumor hyperthermia instrument was used to perform ultrasound hyperthermia (42 ℃-43℃) for 30 min immediately after the injection of PLD drug. High-pressure liquid chromatography (HPLC) was adopted to determine the concentration of doxorubicin in tumor tissues. Results  The temperature of heated zone of the hyperthermia+PLD group was controlled accurately (42.5 ℃±0.2 ℃). The concentration of doxorubicin in tumor tissues of the hyperthermia+PLD group was 2.0 and 11.2 times higher than those of PLD group and doxorubicin group, respectively, and differences were statistically significant (P<0.05). The concentration of doxorubicin in tumor tissues of the PLD group was 5.5 times higher than that of the doxorubicin group and the difference was statistically significant (P<0.05). Conclusion  The combination of embedded ultrasound tumor hyperthermia instrument and PLD can effectively increase the drug concentration of the rabbit VX2 tumor model.

Key words: embedded ultrasound tumor hyperthermia instrument, pegylated liposomal doxorubicin, VX2 tumor model