Abstract:

Objective To assess the biocompatibility of Portland cement (PC). Methods According to GB/T16175 standard, we embedded the PC, mineral trioxide aggregate (MTA), and sterile silicone tube (negative control group) in the back of SD rats subcutaneously. Local inflammation at the interface between materials and tissue was observed 7, 30, and 90 d after embedment. The acute hemolytic experiment was conducted to assess the blood compatibility of materials according to GB/T16886 standard. One-way ANOVA was used to analyze the difference of inflammatory cell numbers among groups and LSD-t was used as multiple comparison methods. Results The PC group, MTA group and negative control group all showed a class Ⅱ inflammation reaction 7 d after embedment. There was no significant difference in inflammatory cell number between the PC group and the MTA group, however，the inflammatory cell number in the negative control group was significantly less than those in two experiment groups. All three groups showed a class Ⅰ inflammation reaction at 30 d, which was alleviated as compared with that seen at 7 d, meanwhile the inflammatory cell numbers in three groups had no significant difference. The inflammatory cells in all groups were very few with no difference between groups at 90 d. The hemolytic experiment showed that hemolysis rates in the PC group and the MTA group were 1.02% and 1.09%, which conformed to the standard of less than 5%. Conclusion It can be preliminarily concluded that PC is an biological material with favorable biocompatibility.

Key words: Portland cement； mineral trioxide aggregate； biocompatible, endodontic disease, treatment material