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Effects of Vitamin D on intra-amniotic lipopolysaccharides-induced alveolarization arrest in neonatal rats

LIU Cheng-bo1, YANG Yi-hui2, LI Wen1, ZHANG Yong-jun1   

  1. 1.Department of Neonatology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2.Department of Pediatrics, Jiading Distinct Central Hospital, Shanghai 201800, China
  • Online:2016-07-28 Published:2016-08-31
  • Supported by:

    National Natural Science Foundation of China, 81270729


Objective To investigate the role of Vitamin D (VitD) on amelioration of chorioamnionitis-induced alveolarization arrest and possible mechanisms. Methods A neonatal rat bronchopulmonary dysplasia (BPD) model was constructed by intra-amniotic injection of lipopolysaccharides (LPS) in pregnant rats. Rats were randomly assigned to the Saline group, LPS+Saline group,LPS+VitD (L) group, and LPS+VitD (H) group. Neonatal rats in the LPS+VitD (L) group and the LPS+VitD (H) group were intraperitoneally injected with 0.5 and 3 ng/g 1, 25(OH)2D3 respectively once a day for 7 d. Meanwhile, neonatal rats in the Saline group and the LPS+Saline group were intraperitoneally injected with the same volume of normal saline. The pulmonary tissues of neonatal rats were harvested 1, 3, and 7 d after the final injection and were stained by H-E staining for observing pathological changes. The mRNA expressions of IL-1β and IFN-γ  were detected using real-time PCR. Results Different concentrations of VitD could improve the birth rate of neonatal rats (P=0.003). The LPS+VitD (L) group and the LPS+VitD (H) group had significantly higher alveolar counts (P=0.001, P=0.000), remarkably decreased mean liner intercept (P=0.000), and significantly decreased mRNA expressions of IL-1β and IFN-γ as compared with the LPS+Saline group 7 d after birth (P=0.000). Conclusion Administration of VitD can significantly decrease the expressions of IL-1β and IFN-γ in lungs of neonatal rats and alleviate the pathological changes of BPD.

Key words: Vitamin D, bronchopulmonary dysplasia, inflammation