›› 2017, Vol. 37 ›› Issue (12): 1616-.doi: 10.3969/j.issn.1674-8115.2017.12.006

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Effects of insulin-like peptide 6 on renal fibrosis and calcification in unilateral ureteral obstruction mice#br#

GAO Lei, WU Shi-yong, JIANG Dan, WANG Ying, LI Yong-yong, WANG De-wei, CHANG Guang-lei, ZHANG Peng, QIN Zheng, ZHANG Dong-ying   

  1. 1. Department of Cardiovascular Medicine, 2. Department of Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Online:2017-12-28 Published:2018-01-10
  • Supported by:
    National Natural Science Foundation of China, 81570212; Chinese Medicine Science and Technology Project of Chongqing Health and Family Planning Commission, ZY201702073; Postgraduate Research and Innovation Project of Chongqing, CYB15093; Cardiac Rehabilitation and Metabolic Therapy Research Foundation; "Advanced" Research Foundation Project of Cardiovascular Health Institute, China Cardiovascular Health Alliance

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Abstract: Objective · To investigate the effects of insulin-like peptide 6 (Insl6) on renal fibrosis and calcification in unilateral ureteral obstruction (UUO) mice.  Methods · Twenty-four SPF male mice with genotypic background of C57BL/6 were divided into Sham (n=8), UUO+saline (n=8) and UUO+Insl6 (n=8) groups randomly. Mice were sacrificed 10 days after operation and renal tissues of surgical side were obtained. Sirus red staining, Masson staining and alizarin red S staining were used to verify the level of collagen and calcium deposition. TGF-β1 expression was determined by Western blotting. Realtime-PCR was used for determining TGF-β1, BMP2, Col1a1, and Col2a1 mRNA expression.  Results · Compared with sham group, fibrotic area especially collagen Ⅰ, calcium deposition, TGF-β1 protein, and TGF-β1, BMP2, Col1a1, and Col2a1 mRNA expression in UUO+saline group significantly
 increased (all P<0.05). As compared with UUO+saline group, fibrotic area especially collagen Ⅰ, calcium deposition, TGF-β1 protein, and TGF-β1,
 BMP2, Col1a1, and Col2a1 mRNA expression in UUO+Insl6 group significantly decreased (all P<0.05).  Conclusion · Insl6 inhibits UUO-induced renal fibrosis and calcification, which may be related to regulation of TGF-β1, collagen Ⅰ , BMP2 and collagen Ⅱ expression levels.

Key words: renal fibrosis, renal calcification, unilateral ureteral obstruction, insulin-like peptide 6