JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) ›› 2021, Vol. 41 ›› Issue (3): 328-333.doi: 10.3969/j.issn.1674-8115.2021.03.007

• Clinical research • Previous Articles     Next Articles

Changes and significance of CX3CR1 expression in peripheral blood monocyte subsets in patients with coronary atherosclerotic heart disease

Qian ZHAO(), Lin GAO, Chang-qian WANG, Jun-feng ZHANG, Hui-li ZHANG, Yang ZHUO()   

  1. Department of Cardiovascular Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Received:2020-04-17 Online:2021-03-28 Published:2021-04-06
  • Contact: Yang ZHUO;
  • Supported by:
    National Natural Science Foundation of China(81570037);Medical guidance;Science and Technology Support Project of Shanghai Science and Technology Commission(19411963300)

Abstract: Objective

·To investigate the expression and clinical significance of C-C motif chemokine receptor 2 (CCR2) and C-X3-C motif chemokine receptor 1 (CX3CR1) in peripheral blood monocytes subsets in patients with different types of coronary atherosclerotic heart disease (CHD).


·A total of 63 CHD patients who underwent coronary angiography (CAG) in Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from January to June 2014 were selected, including 46 patients with acute coronary syndrome (ACS) and 17 patients with stable angina pectoris (SAP). The general data, clinical data and test indexes were collected. Within 24 h after admission, venous blood of the two groups were collected. The expression of CCR2 and CX3CR1 in peripheral blood monocyte subsets was detected by flow cytometry, and monocyte subsets were classified. Serum level of CCL2 (C-C motif chemokine ligand 2) and CX3CL1 (C-X3-C motif chemokine ligand 1) were detected by enzyme-linked immunosorbent assay (ELISA). These patients were followed up for 5 years, and the major adverse cardiac events (MACEs) were recorded.


·Compared with the SAP group, the proportion of CCR2+CX3CR1+ and CCR2-CX3CR1+ monocyte subsets, and the serum levels of CCL2 and CX3CL1 in the ACS group were higher all P<0.05). During the 5-year follow-up, 14 cases in the ACS group and 1 case in the SAP group had MACEs, respectively. Multivariate Logistic regression analysis showed that hypersensitive C-reactive protein level and the proportion of CCR2+CX3CR1- and CCR2-CX3CR1+ monocyte subsets were independent risk factors for the occurrence of MACE in 5 years (all P<0.05).


·Chemokine receptors and chemokines in monocyte subsets alter in different types of CHD. The expression of CX3CR1 of monocyte subsets is possibly related to the long-term outcome of CHD.

Key words: coronary atherosclerotic heart disease (CHD), coronary angiography (CAG), major adverse cardiac event (MACE), chemokine receptor

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