Abstract:

Objective To investigate the changes in proliferation index (PrI) of gingival fibroblasts in nifedipine-induced gingival hyperplasia (NIFr-HGF). Methods Gingival fibroblasts were derived from a patient with nifedipine-induced gingival hyperplasia. Cells were induced by 10 ng/mL and 1 000 ng/mL nifedipine (low- and high-concentration drug intervention groups), respectively. Cells were harvested 18 h and 30 h after intervention, cell cycles were detected by flow cytometry, and PrIs were calculated. NIFr-HGF without nifedipine induction were served as blank control. Results After induction for the same time, PrIs of NIFr-HGF cell cycle of low- and high-concentration drug intervention groups were significantly higher than those of blank control group (P<0.05), while there was no significant difference between low and high-concentration drug intervention groups (P>0.05). In low and high-concentration drug intervention groups, PrIs of NIFr-HGF cell cycle after intervention for 30 h were significantly higher than those after intervention for 18 h [(57.54±0.019)% vs (21.15±0.011)%, and (59.36±0.031)% vs (19.01±0.012)%, respectively] (P<0.05). Conclusion For patients with nifedipine-induced gingival hyperplasia, PrI of NIFr-HGF cell cycle increases with time of nifedipine intervention, while is not significantly related to drug concentration.

Key words: drug-induced gingival hyperplasia, nifedipine, gingival fibroblast, proliferation index