• Original article (Basic research) • Previous Articles     Next Articles

Study on the expression of amyloid precursor protein family in chronic periodontitis

ZHOU Wei1, ZHANG Xuan2, LI Xiao-tian3, ZHANG Jing3, SONG Zhong-chen3   

  1. 1.Shanghai Research Institute of Stomatology, Shanghai Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China; 2.Department of Pharmacy, Shanghai Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; 3.Department of Periodontology, Shanghai Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

  • Online:2016-09-28 Published:2016-10-31
  • Supported by:

    Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support, 20152523; Shanghai Jiao Tong University Cooperation Grant of Medicine, Science, Engineering, YG2015MS07; Chinese Stomatological Asscociation Special Fund, CSA-Y2015-01

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Abstract:

Objective·To observe the expression of amyloid precursor protein (APP) family in chronic periodontitis-affected gingival tissues and in human gingival fibroblasts (HGFs) under inflammatory microenvironment. Methods·Gene expression and protein level of APP family in healthy and chronic periodontitis-affected gingival tissues were measured by real-time PCR and immunohistochemistry (IHC) respectively. HGFs were cultured in vitro. The inflammatory microenvironment was simulated by 1 μg/mL lipopolysaccharide from P. gingivalis (P. g-LPS). The gene expression of APP family in HGFs was also measured using real-time PCR. Results·The gene expression and protein level of APP and APLP2 significantly increased in chronic periodontitis-affected gingival tissues compared with healthy gingival tissues, while the gene expression of APLP1 showed no significant change. The gene expressions of APP, APLP1, and APLP2 in HGFs all increased after being treated with P. g-LPS. Conclusion·APP family may play a role in the occurrence and development of chronic periodontitis.

Key words: amyloid precursor protein family, chronic periodontitis, human gingival fibroblast, inflammatory microenvironment