Abstract:

Objective To analyse the expression pattern of Toll-like receptor (TLR) and main signal transduction factors in tubal epithelial tissues with inflammatory hydrosalpinx. Methods According the results of serum Chlamydia trachomatisIgG antibody testing (CAT), patients with inflammatory hydrosalpinx were divided into CAT positive group (n=20) and CAT negative group (n=8), and normal control group (n=13) was also established. Real-time PCR was employed to detect the expression of TLR1-10 as well as NF-κB, MyD88, TRAF and IRAK mRNA in the fallopian tube epithelial tissues. Results The expression of TLR2 mRNA in CAT positive group and CAT negative group was significantly lower than that in control group (P<0.001), while there was no significant difference between CAT positive group and CAT negative group (P>0.05). The expression of TLR4 mRNA in CAT positive group was significantly lower than that in control group (P<0.001) and CAT negative group (P<0.05), while there was no difference between CAT negative group and control group (P>0.05). The expression of MyD88 in control group was significantly higher than that in CAT positive group (P<0.01) and CAT negative group (P<0.05), while there was no significant difference between CAT positive group and CAT negative group (P>0.05). Conclusion Autoimmunological injury after infection and the reduction of TLR2 and TLR4 in tubal tissues that likely induce an immunological deficiency are associated with hydrosalpinx following a tubal infection.

Key words: hydrosalpinx, Chlamydia trachomatis, IgG, Toll-like receptor, signal transduction