›› 2010, Vol. 30 ›› Issue (5): 535-.

• Original article (Basic research) • Previous Articles     Next Articles

Effects of CTGF antisense oligodeoxynucleotide on transition of human kidney epithelial cells

LIAO Xiao-li1, CHEN Chao1,2, CHEN Tao1,3, YAN Yu-xia1, LIN Chun-lan1, JIANG Jian-wei1   

  1. 1.Department of Biochemistry, Jinan University, Guangzhou 510632, China；2.Department of General Surgery, Jiaozuo People's Hospital, Jiaozuo 454002, China；3.Guangdong Institute of Tuberculosis Prevention and Treatment, Guangzhou 510632, China
  • Online:2010-05-25 Published:2010-05-28
  • Supported by:

    Medical Scientific Research Foundation of Guangdong Province, A2009348

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Abstract:

Objective To investigate the effects of antisense oligodeoxynucleotide (ASODN) targeting connective tissue growth factor (CTGF) on transition of human kidney epithelial cells (HKC). Methods Transition models of HKC were induced by monocyte chemotactic protein-1(MCP-1) and aristolochic acid Ⅰ(AAⅠ). HKC were randomly divided into blank control group, model control group, PEI group, CTGF ASODN 10 ng/mL group and CTGF ASODN 100 ng/mL group. The effects of CTGF ASODN on proliferation and cell cycle of transition models of HKC were evaluated by WST-8 method and flow cytometry, respectively. The expression of α-smooth muscle actin (α-SMA) mRNA and α-SMA, Fibronectin (FN) and TGF-β1 protein of transition models of HKC were detected by RT-PCR and immunohistochemistry, respectively. Results 100 ng/mL CTGF ASODN reversed the proliferation inhibition, reduced S phase arrest, decreased the expression of α-SMA mRNA and α-SMA, FN and TGF-β1 protein of HKC induced by MCP-1 and AAⅠ. Conclusion CTGF ASODN can inhibit the transition of HKC induced by MCP-1 and AAⅠ.

Key words: connective tissue growth factor, antisense oligodeoxynucleotide, epithelial-to-mesenchymal transition, human kidney epithelial cell