Abstract:

Objective To explore the mechanism of low-dose chemotherapy in combination with dendritic cell (DC) vaccine in treatment of murine lung cancer. Methods Lewis lung cancer cells (3LL) were inoculated subcutaneously in mice. Mice were divided into four groups (n=6) after tumor formation: control group (no treatment), low-dose chemotherapy group (treated with Paclitaxel 2 mg/kg), DC vaccine group and combined treatment group (treated with low-dose Paclitaxel and DC). On day 21, interferon-γ-induced protein 10 (IP-10), monocyte chemoattractant protein-1(MCP-1), keratinocyte (KC) and interleukin-1α (IL-1α) in intratumoral and peritumoral tissues were detected by microdialysis combined with Luminex technology in mice. Results Compared with the other three groups, the production of IP-10, MCP-1 and KC at some different time points was significantly higher in combined treatment group (P<0.05), while there was no significant difference in the production of IP-10, MCP-1, KC and IL-1α in peritumoral tissues among these four groups (P>0.05). Conclusion The combination of low-dose chemotherapy with DC vaccine may inhibit the growth of murine lung cancer,and the mechanism probably relates to regulation of microenviroment in the tumors.

Key words: dendritic cell, lung cancer, chemomodulation