›› 2010, Vol. 30 ›› Issue (5): 570-.

• Original article (Clinical research) • Previous Articles     Next Articles

Relationship between expression of PHDs mRNA and Bax and Bcl-2 in non-small cell lung cancer

CHEN Su-feng1, ZHANG Jie2, ZHU Xiao-li3, YAO Shi-hua1, ZHANG Jun-hua1, CHEN Hai-quan2   

  1. 1.Department of Cardiothoracic Surgery, The Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China；2.Department of Thoracic Surgery, 3.Department of Pathology, Shanghai Cancer Center, Fudan University, Shanghai 200032, China
  • Online:2010-05-25 Published:2010-05-28

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Abstract:

Objective To investigate the expression of prolyl hydroxylase domain enzymes genes (PHDs mRNA), and explore its relationship with expression of apoptosis-related protein Bax and Bcl-2 in non-small cell lung cancer. Methods The expression of PHD1, 2, 3 mRNA and Bax and Bcl-2 in cancer tissues and para-cancerous normal tissues in 56 cases of non-small cell lung cancer was detected by Real-time PCR and immunohistochemical staining, respectively. Results The expression of PHDs mRNA in cancer tissues was significantly higher than that in para-cancerous normal tissues (P<0.05). Patients with positive expression of Bax and Bcl-2 accounted for 33.9% (19/56) and 32.1% (18/56), respectively. The positive expression rates of Bcl-2 in cancer tissues with high expression and low expression of PHD3 mRNA were 19.5% and 66.7%, respectively. Conclusion The expression of PHDs mRNA is increased in nonsmall cell lung cancer tissues, and the increased expression of PHD3 mRNA may be associated with cell apoptosis.

Key words: prolyl hydroxylases, non-small cell lung cancer, apoptosis, Bcl-2, Bax