Abstract:

Objective To evaluate the thrombolytic effects of magnetic nano-mesoporous materials carrying urokinase in flow conditions. Methods Thrombosis was prepared and linked to flow model in vitro. Four groups were divided, and were treated with normal saline (blank control group), urokinase (urokinase control group), magnetic urokinase (magnetic urokinase group) and magnetic urokinase plus external magnetic field (magnetic urokinase plus external magnetic field group), respectively. The weight of thrombus before and after thrombolysis was obtained, the rates of thrombolysis were calculated, and the amount of liquid recanalization was recorded. Results Magnetic urokinase aggregation in the target position was clearly observed in magnetic urokinase plus external magnetic field group. There was no significant difference in the rates of thrombolysis between urokinase control group (76.89%±0.048%) and magnetic urokinase group (75.42%±0.094%) (P>0.05), while the rate of thrombolysis in magnetic urokinase plus external magnetic field group (90.45%±0.076%) was significantly higher than those in urokinase control group and magnetic urokinase group (P<0.05). There was no significant difference in the amount of liquid recanalization between urokinase control group [(42.47±1.834) mL)] and magnetic urokinase group [(39.70±5.028) mL] (P>0.05), while the amount of liquid recanalization in magnetic urokinase plus external magnetic field group [(60.29±3.216)mL] was significantly higher than that in urokinase control group and magnetic urokinase group (P<0.05). Conclusion The magnetic nano-mesoporous material as a carrier of thrombolytic drugs can enhance the targeting of urokinase, and improve the rate of thrombosis in flow conditions.

Key words: magnetic nano-mesoporous materials, urokinase, thrombolysis in vitro