Abstract:

Objective To investigate the effects of different resuscitation fluids on coagulation function of normovolemic hemodilution and autologous blood transfusion after hepatic hemorrhage. Methods Models of normovolemic hemodilution and autologous blood resuscitation after hepatic hemorrhage were established in 18 New Zealand rabbits, and were randomly divided into H group (Hydroxyethyl starch 130/0.4), G group (Gelofusine) and RL group (Ringer lactate) according to different resuscitation fluids (n=6 in each group). Blood samples were collected from femoral arteries after anesthesia and before hemodilution (T0), 10 min after normovolemic dilution (T1) and 60 min after autologous blood resuscitation after hepatic hemorrhage (T2). Coagulation function was tested, and thromboelastograph (TEG) measurements were conducted. Besides, time of survival and volumes of blood loss were monitored in each group within 120 min of resuscitation after hepatic hemorrhage. Results Parameters of coagulation function at T1 and T2 time points were significantly different from those at T0 time point in H group （P<0.05）. There were also significant differences in TEG measurements between T1 time point and T2 time point in H group （P<0.05）. Time of survival was shorter, and volume of blood loss was larger in H group. There was a decrease tendency of mean artery pressure (MAP) during autologous blood transfusion after hepatic hemorrhage in H group, and was significantly different from those of G group and RL group （P<0.05）. Conclusion Hydroxyethyl starch 130/0.4 has a greater inhibitive effect on coagulation function than Gelofusine and Ringer lactate.

Key words: normovolemic hemodilution, resuscitation, autologous blood transfusion after hepatic hemorrhage