Abstract:

Objective To culture and purify the mouse satellite cells of gastrocnemius and soleus muscles in vitro, and detect the expression of β-actin in cells. Methods Skeletal muscle satellite cells of gastrocnemius and soleus muscles of ICR mice were purified by collagenase and trypsin digestion and differential adhesion method, and were identified by immunofluorescent antibody cytochemical staining. Total RNA was extracted from the second generation of gastrocnemius and soleus muscles, and semiquantitative RT-PCR was performed to detect the relative expression of β-actin gene with GAPDH as an internal standard. Results The expression of fast- and slow-myosin was positive in satellite cells of gastrocnemius and soleus cultured in vitro, with favorable cell growth and proliferation. The relative expression of β-actin gene in gastrocnemius and soleus muscles was 3.71072 and 2.106028, respectively (P<0.01). Conclusion The expression of β-actin in gastrocnemius is higher than that in soleus muscle, which suggests the expression of β-actin may be related to the types of muscle fibers.

Key words: β-actin, satellite cell, in vitro, skeletal muscle, gastrocnemius, soleus muscle, mouse