›› 2012, Vol. 32 ›› Issue (7): 861-.doi: 10.3969/j.issn.1674-8115.2012.07.009

• Original article (Basic research) • Previous Articles     Next Articles

Regulation of inflammatory response in monocytes by PPARgamma agonist

WANG Jing1, XU Ping1, HOU Yan-qiang2, LOU Xiao-li2, HUANG Ling1   

  1. 1.Department of Gastroenterology, 2.Central Laboratory, Songjiang Central Hospital, the First People´s Hospital, Shanghai Jiaotong University, Shanghai 201600, China
  • Online:2012-07-28 Published:2012-08-17
  • Supported by:

    Natural Science Foundation of Shanghai, 09ZR1429100；Shanghai Songjiang District Health Bureau Foundation, 201101

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Abstract:

Objective To investigate the effects of peroxisome proliferator-activated receptor-gamma (PPARγ) agonist on inflammatory response in monocytes. Methods Human monocytes THP-1 cells were cultured in vitro, and were divided into control group, experiment group, pioglitazone group and GW9662 group. Cells in control group were treated with culture fluid, cells in experiment group were stimulated by pancreatitis associated ascetic fluid (PAAF), 20 μmol/L PPARγ agonist pioglitazone was used before treatment with PAAF in pioglitazone group, and cells were treated with PPARγ antagonist GW9662, pioglitazone and PAAF in order in GW9662 group. Cells in each group were collected 6 h after treatment, Real-Time PCR was employed to detect the expression of proinflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) mRNA, and the expression of PPARγ mRNA and protein was determined by RT-PCR and Western blotting respectively. Results After stimulation with PAAF, the relative expression of TNF-α and IL-6 mRNA was significantly higher, and the relative expression of PPARγ mRNA was significantly lower in experiment group, pioglitazone group and GW9662 group than in control group (P<0.05). The relative expression of TNF-α and IL-6 mRNA was significantly lower, and the relative expression of PPARγ mRNA was significantly higher in pioglitazone group and GW9662 group than in experiment group (P<0.05). The relative expression of TNF-α and IL-6 mRNA was significantly higher, and the relative expression of PPARγ mRNA was significantly lower in GW9662 group than in pioglitazone group (P<0.05). The change of expression of PPARγ protein was in line with that of PPARγ mRNA in each group, and there was no significant difference in the expression of PPARγ protein between pioglitazone group and control group and between GW9662 group and pioglitazone group (P＞0.05). Conclusion PPARγ agonist may up-regulate the expression of PPARγ mRNA, decrease the production of proinflammatory cytokines stimulated by PPAF, and inhibit the inflammation in monocytes.

Key words: peroxisome proliferator-activated receptors gamma, peroxisome proliferator-activated receptors gamma agonist, acute pancreatitis, pancreatitis associated ascetic fluid