Abstract:

Objective To investigate the effect of hypothermia on the expression of calcium and integrin-binding protein 1 (CIB1) in CA1 region of hippocampus after traumatic brain injury (TBI) in rats. Methods Fifty-four SD rats were randomly assigned to hypothermia group (n=24), normothermia group (n=24) and sham injury group (n=6). TBI models were established in hypothermia group and normothermia group, and hypothermia intervention (32 ℃，4 h) was conducted in hypothermia group. Rats in hypothermia group and normothermia group were sacrificed 4 h, 6 h, 12 h and 24 h after TBI, and those in sham injury group were sacrificed 24 h after injury, with 6 rats at each time point. Tissues in CA1 region of the injury side were obtained, and RT-PCR and Western blotting were adopted to detect the expression of CIB1 mRNA and protein. Results The expression of CIB1 mRNA and protein in CA1 region of hippocampus of the injury side in hypothermia group and normothermia group was significantly higher than that in sham injury group at each time point after injury (P<0.05), and the expression reached the peak at the time points of 4 h and 6 h after TBI. The expression of CIB1 mRNA and protein in hypothermia group was significantly lower than that in normothermia group (P<0.05). Conclusion Hypothermia treatment can suppress the upregulation of expression of CIB1 mRNA and protein in hippocampus after TBI in rats.

Key words: traumatic brain injury, hypothermia, calcium and integrin-binding protein 1