Objective To construct the acellular artificial dermis loaded with nanoparticles that contain different ratios of platelet-derived growth factor-BB (PDGF-BB) and insulin-like growth factor-1 (IGF-1) for repairing skin wounds of nude mice and to explore the best ratio of PDGF-BB and IGF-1 for repairing skin wounds. Methods Sustained released nanoparticles were made by enveloping different ratios of PDGF-BB and IGF-1 with polylactic acid-glycolic acid (PLGA) and then were combined with acellular artificial dermis. Nanoparticle loaded acellular artificial dermis was transplanted to the wound model of nude mice. Effects of nanoparticle loaded acellular artificial dermis of groups with different mass ratio of PDGF-BB and IGF-1 (1∶4, 1∶2, 1∶1, 2∶1, and 4∶1)(n=5) on healing mice skin wounds were observed by recording the wound healing speed and the average healing time and by detecting the protein expressions of α-SMA and IL-2 in local tissues. In addition, the blank control group was also established. Results When the mass ratio of PDGF-BB and IGF-1 of nanoparticle loaded acellular artificial dermis was 2:1, the wound healing speed was faster than that of the blank control group and groups with other mass ratios (P<0.05); the average healing time was shorter (P<0.05); and the protein expressions of α-SMA and IL-2 in local tissues were higher (P<0.05). Conclusion When acellular artificial dermis loaded with nanoparticles that contain PDGF-BB and IGF-1 is used for skin wound healing, the best ratio of PDGF-BB and IGF-1 is 2∶1.