Objective·To measure the expression of ST20-AS1, a long non-coding RNA (lncRNA) in patients with T-cell lymphoma, and to explore its effects on the proliferation and invasion of T-cell lymphoma cells. Methods·Study lncRNA was screened using the lncRNA array. The association between high expression of ST20-AS1 and NOTCH signaling pathway was detected via the data from the lncRNA array. The expression level of ST20-AS1 in T-cell lymphoma patients was assessed by real-time RT-PCR and its activation effect on NOTCH signaling pathway was verified. ST20-AS1-overexpressing T-cell lymphoma cell line Jurkat was constructed and cell proliferation test and Transwell assay were performed to observe changes in cell proliferation and invasion. Results·ST20-AS1 was overexpressed and NOTCH signaling pathway was significantly activated in T-cell lymphoma patients. The overexpression of ST20-AS1 activated the NOTCH signaling pathway and promoted lymphoma cell proliferation and invasion in Jurkat. When NOTCH1 was knocked down by siRNA, cell proliferation and invasion was reduced in ST20-AS1-overexpressing cell line Jurkat. Conclusion·ST20-AS1 can activate the NOTCH signaling pathway and promote lymphoma cell proliferation and invasion.