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Expression and biological effect of long non-coding RNA  ST20-AS1  in T-cell lymphoma

SUN Yi-feng, XU Peng-peng, ZHAO Wei-li   

  1. Shanghai Institute of Hematology, Department of Hematology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine， Shanghai  200025, China
  • Online:2016-09-28 Published:2016-10-31
  • Supported by:

    National Natural Science Foundation of China, 81325003, 81520108003, 81201863; Shanghai Commission of Science and Technology, 14430723400, 14140903100； Ruijin Hospital Outstanding Young Teachers, 800000000003； Shanghai Jiao Tong University Morningstar Award； Shanghai Young Doctor Training Program； Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support， 20152208

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Abstract:

Objective·To measure the expression of ST20-AS1, a long non-coding RNA (lncRNA) in patients with T-cell lymphoma, and to explore its effects on the proliferation and invasion of T-cell lymphoma cells. Methods·Study lncRNA was screened using the lncRNA array. The association between high expression of ST20-AS1 and NOTCH signaling pathway was detected via the data from the lncRNA array. The expression level of ST20-AS1 in T-cell lymphoma patients was assessed by real-time RT-PCR and its activation effect on NOTCH signaling pathway was verified. ST20-AS1-overexpressing T-cell lymphoma cell line Jurkat was constructed and cell proliferation test and Transwell assay were performed to observe changes in cell proliferation and invasion. Results·ST20-AS1 was overexpressed and NOTCH signaling pathway was significantly activated in T-cell lymphoma patients. The overexpression of ST20-AS1 activated the NOTCH signaling pathway and promoted lymphoma cell proliferation and invasion in Jurkat. When NOTCH1 was knocked down by siRNA, cell proliferation and invasion was reduced in ST20-AS1-overexpressing cell line Jurkat. Conclusion·ST20-AS1 can activate the NOTCH signaling pathway and promote lymphoma cell proliferation and invasion.

Key words: T-cell lymphoma, ST20-AS1, NOTCH signaling pathway, cell proliferation, cell invasion