Journal of Shanghai Jiao Tong University (Medical Science) >

2017 , Vol. 37 >Issue 4: 566

DOI: https://doi.org/10.3969/j.issn.1674-8115.2017.04.029

Review

Research progresses of applying MALDI-TOF mass spectrometry in the detection of β-lactamase

  • YU Jia-jia ,
  • YU Jing ,
  • LIU Ying
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  • Department of Clinical Microbiology, Clinical Laboratory, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China

Online published: 2017-05-04

Supported by

Shanghai Shenkang Hospital Development Center Clinical Auxiliary Departments Capacity Building Project，SHDC22014003

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Abstract

Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is an emerging tool for detecting microorganisms. It can be used not only for rapid identification of microorganisms, but also for the research of resistance mechanisms. Producing β-lactamase is the main mechanism for resistance of β-lactam antibiotics in Gram-negative bacilli. Currently using MALDI-TOF MS for rapid detection of bacterial β-lactamase has been widely reported, including detection of β-lactam hydrolysis activity of β-lactamase, direct detection of β-lactamase molecule, peptide and relevant proteins, and detection of the single nucleotide polymorphisms (SNPs) of β-lactamase gene. This paper reviews current application of MALDI-TOF MS to the detection of β-lactamase based on the latest research findings.

Key words： matrix assisted laser desorption ionization time of flight mass spectrometry; β-lactamase; hydrolysis test; single nucleotide polymorphisms

Cite this article

YU Jia-jia , YU Jing , LIU Ying . Research progresses of applying MALDI-TOF mass spectrometry in the detection of β-lactamase[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2017 , 37(4) : 566 . DOI: 10.3969/j.issn.1674-8115.2017.04.029

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