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Research progresses of applying MALDI-TOF mass spectrometry in the detection of β-lactamase

YU Jia-jia, YU Jing, LIU Ying   

  1. Department of Clinical Microbiology, Clinical Laboratory, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China
  • Online:2017-04-28 Published:2017-05-04
  • Supported by:

    Shanghai Shenkang Hospital Development Center Clinical Auxiliary Departments Capacity Building Project,SHDC22014003


Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is an emerging tool for detecting microorganisms. It can be used not only for rapid identification of microorganisms, but also for the research of resistance mechanisms. Producing β-lactamase is the main mechanism for resistance of β-lactam antibiotics in Gram-negative bacilli. Currently using MALDI-TOF MS for rapid detection of bacterial β-lactamase has been widely reported, including detection of β-lactam hydrolysis activity of β-lactamase, direct detection of β-lactamase molecule, peptide and relevant proteins, and detection of the single nucleotide polymorphisms (SNPs) of β-lactamase gene. This paper reviews current application of MALDI-TOF MS to the detection of β-lactamase based on the latest research findings.

Key words: matrix assisted laser desorption ionization time of flight mass spectrometry, β-lactamase, hydrolysis test, single nucleotide polymorphisms