›› 2017, Vol. 37 ›› Issue (8): 1069-.doi: 10.3969/j.issn.1674-8115.2017.08.004

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Isolation and characterization of bacteriophage against extended-spectrum β-lactamase-producing Escherichia coli strains

HUANG Lei-jie1*, LIU Xiao-liang1*, ZHU Rui1, LIN Fang-qi1, QIN Jin-hong2, HE Ping2, GUO Xiao-kui2, ZHAO Wei1   

  1. 1. Experiment Teaching Center of Basic Medicine, 2. Department of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2017-08-28 Published:2017-09-28
  • Supported by:
    Shanghai Three-year Plan of the Key Subjects Construction in Public Health-Infectious Diseases and Pathogenic Microorganism, 15GWZK0102

Abstract: Objective · To isolate phages which can fight against extended-spectrum β-lactamase (ESBLs)-producing Escherichia coli (E. coli), and provide basic research for establishment of E. coli phage library and treatment of bacterial infection.  Methods · Samples collected from sewage were co-cultured with 93 ESBLs-producing E. coli strains. A phage named JDEC001 was isolated by double agar overlay plaque assay. The biological characteristics, complete genome sequence and comparative genome analyses of JDEC001 were studied respectively.  Results · JDEC001 belongs to the lytic phage as a member of the Caudovirales order, Podoviridae family. It has high activity at pH from 5 to 11 and with temperature from 0 to 39 ℃ . Whole-genome sequencing of JDEC001 demonstrated double-stranded DNA genome of 38 745 bp with GC content of 49.93%, which encoded 46 open reading frames. The comparative genomics also showed that there was no virulent genes or antibiotic resistant genes in its genome.  Conclusion · The phage JDEC001 against ESBLs-producing E. coli was isolated and purified, with good stability in a broad range of pH and temperature.

Key words:  extended-spectrum β-lactamase-producing Escherichia coli, phages, biological characteristics, genomic characteristics