Journal of Shanghai Jiao Tong University (Medical Science) >

2017 , Vol. 37 >Issue 12: 1623

DOI: https://doi.org/10.3969/j.issn.1674-8115.2017.12.007

Preliminary study of flavonoids affecting cAMP level in cannabinoid receptor overexpressed cells#br#

  • LI Ting ,
  • LIU Jian-hua
Expand
  • 1. Department of Chemistry, Basic Medicine Faculty of Shanghai Jiao Tong University, Shanghai 200025, China; 2. Department of Pharmacy, Shanghai Pudong New District Zhoupu Hospital, Shanghai 201318, China

Online published: 2018-01-10

Supported by

National Natural Science Youth Fund of China, 81402775； Special Research Fund for Doctoral Programs in Colleges and Universities, 20130073120113

Fold

Abstract

Objective · To evaluate the effects of flavonoids on cyclic adenosine monophosphate (cAMP) level in cannabinoid receptor (CBR) overexpressed cells.  Methods · Eukaryotic expression vectors GV144-CB1R and GV144-CB2R were constructed, and transfected into HEK-293 cells to establish CBR overexpressed cell lines. Several flavonoid compounds were studied by measuring the change of cAMP level in CBR-overpressed HEK293 cell lines.  Results · The CBR overexpressed cell lines were constructed successfully. cAMP level in CBR-overexpressed HEK-293 cells decreased after incubation with flavonoid compounds. And these effects were selective for CB2R overexpressed cells.  Conclusion · All flavonoids studied exhibited decreasing effect on cAMP level in CBR-overexpressed cells at μmol/L level.

Key words： cannabinoid receptor; flavonoids/flavonoid compounds; cannabinoid receptor overexpressed cell; cyclic adenosine monophosphate

Cite this article

LI Ting , LIU Jian-hua . Preliminary study of flavonoids affecting cAMP level in cannabinoid receptor overexpressed cells#br#[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2017 , 37(12) : 1623 . DOI: 10.3969/j.issn.1674-8115.2017.12.007

Options
Outlines

/

〈 〉