›› 2017, Vol. 37 ›› Issue (12): 1623-.doi: 10.3969/j.issn.1674-8115.2017.12.007

Previous Articles     Next Articles

Preliminary study of flavonoids affecting cAMP level in cannabinoid receptor overexpressed cells#br#

LI Ting1, 2, LIU Jian-hua1   

  1. 1. Department of Chemistry, Basic Medicine Faculty of Shanghai Jiao Tong University, Shanghai 200025, China; 2. Department of Pharmacy, Shanghai Pudong New District Zhoupu Hospital, Shanghai 201318, China
  • Online:2017-12-28 Published:2018-01-10
  • Supported by:
    National Natural Science Youth Fund of China, 81402775; Special Research Fund for Doctoral Programs in Colleges and Universities, 20130073120113

Abstract: Objective · To evaluate the effects of flavonoids on cyclic adenosine monophosphate (cAMP) level in cannabinoid receptor (CBR) overexpressed cells.  Methods · Eukaryotic expression vectors GV144-CB1R and GV144-CB2R were constructed, and transfected into HEK-293 cells to establish CBR overexpressed cell lines. Several flavonoid compounds were studied by measuring the change of cAMP level in CBR-overpressed HEK293 cell lines.  Results · The CBR overexpressed cell lines were constructed successfully. cAMP level in CBR-overexpressed HEK-293 cells decreased after incubation with flavonoid compounds. And these effects were selective for CB2R overexpressed cells.  Conclusion · All flavonoids studied exhibited decreasing effect on cAMP level in CBR-overexpressed cells at μmol/L level.

Key words: cannabinoid receptor, flavonoids/flavonoid compounds, cannabinoid receptor overexpressed cell, cyclic adenosine monophosphate