Hippo signaling pathway exists in drosophila and mammals, which is mainly composed of upstream modulators, core components and downstream modulators and has physiological functions such as cell proliferation, differentiation and cell cycle. In recent years, some research has shown that the Hippo signaling pathway can participate in three major substances, including glucose metabolism, mevalonate metabolism and glutamine metabolism, either alone or in combination with the AMP-activated protein kinase (AMPK) signaling pathway and the mammalian target of rapamycin (mTOR) signaling pathway. The relationship between Hippo signaling pathway and ovarian material metabolism is a research hotspot that has emerged in recent years. In this review, we summarize research progress that the Hippo signaling pathway regulates the maturation of follicles, polycystic ovary syndrome and ovarian cancer, through either alone or in combination with the AMPK signaling pathway and the mTOR signaling pathway.