Journal of Shanghai Jiao Tong University (Medical Science) >

2021 , Vol. 41 >Issue 7: 884 - 890

DOI: https://doi.org/10.3969/j.issn.1674-8115.2021.07.006

Basic research

Effect of miR-877-3p on proliferation of bone marrow mesenchymal stem cells in osteoporosis

  • Xu TONG ,
  • Lin-jing SHU
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  • 1.Department of Prosthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China
    2.Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China
    3.Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
    4.Department of Implant, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China

Online published: 2021-08-03

Supported by

National Natural Science Foundation of China for Young Scholars(81800979)

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Abstract

Objective

·To investigate the effect of miR-877-3p on the proliferation of bone marrow mesenchymal stem cells (BMSCs) during the occurrence and development of osteoporosis.

Methods

·Twenty female healthy mice aged 8 weeks underwent bilateral ovariectomy to establish the postmenopausal osteoporosis models (OVX group). In addition, 20 healthy female mice were selected in the same period and the adipose tissue near the ovary was removed to establish sham operation models (Sham group). Two months after surgery, bone parameters of the mice, including bone volume fraction (BVF), trabecular number (Tb.N), and bone mineral density (BMD) were detected by micro-CT, and cell proliferation ability of the two groups was detected by CCK-8 and cell counting. The expression of miR-877-3p in the two groups was detected by real-time PCR. After up-regulating (down-regulating) the level of miR-877-3p in the BMSCs, cell proliferation was detected by CCK-8 and cell counting.

Results

·The micro-CT results showed that there were significant differences in bone parameters between the Sham group and the OVX group (P<0.05). The results of CCK-8 and cell counting showed that the proliferation ability of BMSCs in the Sham group was significantly higher than that in the OVX group (P<0.05). Real-time PCR results showed that the expression of miR-877-3p in BMSCs in the Sham group was significantly lower than that in the OVX group (P<0.05). After upregulation (downregulation) of miR-877-3p, the proliferation ability of BMSCs decreased (increased).

Conclusion

·In postmenopausal osteoporosis mice, the proliferation ability of BMSCs is regulated by miR-877-3p, which may be one of the important factors affecting the incidence of osteoporosis.

Key words： bone mesenchymal stem cells; proliferation; miR-877-3p; menopause

Cite this article

Xu TONG , Lin-jing SHU . Effect of miR-877-3p on proliferation of bone marrow mesenchymal stem cells in osteoporosis[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2021 , 41(7) : 884 -890 . DOI: 10.3969/j.issn.1674-8115.2021.07.006

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