Journal of Shanghai Jiao Tong University (Medical Science) >

2022 , Vol. 42 >Issue 3: 307 - 311

DOI: https://doi.org/10.3969/j.issn.1674-8115.2022.03.007

Basic research

Effect of decreased 5⁃hydroxytryptamine synthesis during puberty development on the onset of puberty in female rats

  • Ting DONG ,
  • Xiaoqin YIN ,
  • Yan LI ,
  • Pin LI
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  • Department of Endocrinology, Shanghai Children's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200062, China
LI Pin, E-mail: lipin21@126.com.

Received date: 2021-12-16

  Online published: 2022-05-09

Supported by

National Natural Science Foundation of China(81871131);Sailing Program of Science and Technology Commission of Shanghai Municipality(20YF1440700)

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Abstract

Objective

·To preliminarily explore the effects of decreased 5-hydroxytryptamine (5-HT) synthesis in the hypothalamus during puberty development on the onset of puberty in female rats.

Methods

·Seventy two 21-day-old female SD rats were randomly divided into para-chlorophenylalanine (PCPA) group and normal saline group (control group), with 36 rats in each group. The rats in the PCPA group were injected with PCPA intraperitoneally every day (to establish the model of decreased 5-HT synthesis), while the rats in the control group were injected with the same amount of normal saline. The administration treatment lasted until 1 d before death. The rats were sacrificed at the age of 28 days, 35 days and 42 days, respectively. The expression of gonadotropin-releasing hormone (GnRH) gene in the hypothalamus of the two groups was detected by quantitative real-time PCR (qPCR). The content of 5-HT and GnRH in the hypothalamus and the level of luteinizing hormone (LH) in peripheral blood were detected by enzyme-linked immunosorbent assay (ELISA). At the same time, since the age of 28 days, the vaginal opening time in the two groups was observed and recorded from 9:00 to 10:00.

Results

·The ELISA results of 5-HT content showed that the rats in the control group had the lowest level at 28 days and the highest level at 42 days, and showed an overall increasing trend, which was consistent with the trend of the PCPA group. Compared with the control group, the content of 5-HT in the PCPA group was lower at the above three time points (all P<0.05). qPCR and ELISA results of GnRH content showed that compared with the control group, the expressions of GnRH mRNA and GnRH protein in the PCPA group were lower at the above three time points (all P<0.05). The results of ELISA showed that compared with the control group, LH levels of the PCPA group were lower at the age of 28 days and 35 days, and the difference was statistically significant (both P<0.05). The observation results showed that compared with the control group, vaginal opening time of the PCPA group was delayed (P=0.035).

Conclusion

·The decrease of 5-HT synthesis during puberty development can lead to decrease of GnRH synthesis in the hypothalamus, decrease of LH level in peripheral blood and delay of vaginal opening, thus delaying the onset of puberty in female SD rats.

Key words： 5-hydroxytryptamine (5-HT); rat; puberty development; gonadotropin-releasing hormone (GnRH)

Cite this article

Ting DONG , Xiaoqin YIN , Yan LI , Pin LI . Effect of decreased 5⁃hydroxytryptamine synthesis during puberty development on the onset of puberty in female rats[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2022 , 42(3) : 307 -311 . DOI: 10.3969/j.issn.1674-8115.2022.03.007

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