Journal of Shanghai Jiao Tong University (Medical Science) >

2022 , Vol. 42 >Issue 9: 1216 - 1224

DOI: https://doi.org/10.3969/j.issn.1674-8115.2022.09.008

Innovative research team achievement column

Anatomical structure of laminar specific subtypes of medial prefrontal cortex-basolateral amygdala projection neurons

  • Luyao HE ,
  • Dongping HUANG ,
  • Mengmeng SHAO ,
  • Kai ZHANG ,
  • Baihui REN ,
  • Qingdan KONG ,
  • Tianle XU ,
  • Jiangteng Lü
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  • 1.Department of Anatomy and Physiology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China
    2.Center for Brain Science of Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
XU Tianle, E-mail: xu-happiness@shsmu.edu.cn.
LÜ Jiangteng, E-mail: jiangtenglu@shsmu.edu.cn

Received date: 2022-01-27

  Accepted date: 2022-06-07

  Online published: 2022-09-08

Supported by

National Natural Science Foundation of China(31972903);Innovative Research Team of High-Level Local Universities in Shanghai(SSMU-ZDCX20181100);Basic Research Project of Science and Technology Commission of Shanghai Municipality(18JC1420302)

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Abstract

Objective ·To dissect the fine anatomical structure of medial prefrontal cortex (mPFC)-basolateral amygdala (BLA) projection neurons based on regional and laminar specificity in the cortex in mice. Methods ·The retrograde tracer of adeno-associated viral (AAV) vectors were injected into BLA of C57BL/6 mice. Twenty-one days after the injection, the mice were sacrificed and the brains were harvested. The coronal sections containing mPFC were prepared by using a cryostat microtome. The images were taken by fluorescence microscopy. Then the representative brain slices containing mPFC were selected and the distribution of virus-labeled cell bodies in different subregions of mPFC was analyzed. By using transgenic mouse lines (Tbr2-CreER::LSL-Flp mice and Rbp4-Cre mice)with Flp- or Cre-dependent AAV, layer 2 (L2) and layer 5 (L5) projection neurons in mPFC and their axons in BLA were marked, respectively. Twenty-eight days after the injection, the mice were sacrificed and the brains were also harvested. Coronal sections of the BLA were collected. The brain slices were imaged by fluorescence microscopy after immunofluorescence staining. By measuring the fluorescence intensity of projecting axons in different regions within BLA, the spatial distributions of the axons in BLA of mPFC L2 and L5 projection neurons were compared. Results ·Through fluorescence microscopy and quantitative analysis, it was found that the cell bodies of BLA projection neurons were mainly located at L2 and L5 in the dorsal mPFC (dmPFC) which contains anterior cingulate cortex and prelimbic cortex. However, the distribution of BLA projection neurons in the ventral mPFC (vmPFC) including medial orbital cortex and infralimbic cortex did not exhibit the similar distribution. When studying the axonal distribution of dmPFC projection neurons in two layers, it was found that the axons of L2 projection neurons dispersed in the BLA, while the axons of L5 projection neurons were mainly located in the dorsal region of BLA. Conclusion ·The cell body distribution of mPFC-BLA projection neurons shows regional specificity and laminar specificity. The axon projections initiated from different layers of dmPFC also exhibit regional specificity in BLA.

Key words： projection neuron; medial prefrontal cortex (mPFC); basolateral amygdala (BLA); regional specificity; laminar specificity; axon

Cite this article

Luyao HE , Dongping HUANG , Mengmeng SHAO , Kai ZHANG , Baihui REN , Qingdan KONG , Tianle XU , Jiangteng Lü . Anatomical structure of laminar specific subtypes of medial prefrontal cortex-basolateral amygdala projection neurons[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2022 , 42(9) : 1216 -1224 . DOI: 10.3969/j.issn.1674-8115.2022.09.008

References

1 MILLER E K, COHEN J D. An integrative theory of prefrontal cortex function[J]. Annu Rev Neurosci, 2001, 24: 167-202.
2 ROBBINS T W, ARNSTEN A F T. The neuropsychopharmacology of fronto-executive function: monoaminergic modulation[J]. Annu Rev Neurosci, 2009, 32: 267-287.
3 LAUBACH M, AMARANTE L M, SWANSON K, et al. What, if anything, is rodent prefrontal cortex? [J]. eNeuro, 2018, 5(5): ENEURO.0315-18.2018.
4 ANASTASIADES P G, CARTER A G. Circuit organization of the rodent medial prefrontal cortex[J]. Trends Neurosci, 2021, 44(7): 550-563.
5 SCHUMAN B, MACHOLD R P, HASHIKAWA Y, et al. Four unique interneuron populations reside in neocortical layer 1[J]. J Neurosci, 2019, 39(1): 125-139.
6 ABS E, POORTHUIS R B, APELBLAT D, et al. Learning-related plasticity in dendrite-targeting layer 1 interneurons[J]. Neuron, 2018, 100(3): 684-699.e6.
7 ANASTASIADES P G, COLLINS D P, CARTER A G. Mediodorsal and ventromedial thalamus engage distinct L1 circuits in the prefrontal cortex[J]. Neuron, 2021, 109(2): 314-330.e4.
8 JANAK P H, TYE K M. From circuits to behaviour in the amygdala[J]. Nature, 2015, 517(7534): 284-292.
9 BLAIR H T, HUYNH V K, VAZ V T, et al. Unilateral storage of fear memories by the amygdala[J]. J Neurosci, 2005, 25(16): 4198-4205.
10 SAH P, FABER E S L, LOPEZ DE ARMENTIA M, et al. The amygdaloid complex: anatomy and physiology[J]. Physiol Rev, 2003, 83(3): 803-834.
11 FERRARA N C, TRASK S, ROSENKRANZ J A. Maturation of amygdala inputs regulate shifts in social and fear behaviors: a substrate for developmental effects of stress[J]. Neurosci Biobehav Rev, 2021, 125: 11-25.
12 GANGOPADHYAY P, CHAWLA M, DAL MONTE O, et al. Prefrontal-amygdala circuits in social decision-making[J]. Nat Neurosci, 2021, 24(1): 5-18.
13 ARRUDA-CARVALHO M, CLEM R L. Pathway-selective adjustment of prefrontal-amygdala transmission during fear encoding[J]. J Neurosci, 2014, 34(47): 15601-15609.
14 BUKALO O, PINARD C R, SILVERSTEIN S, et al. Prefrontal inputs to the amygdala instruct fear extinction memory formation[J]. Sci Adv, 2015, 1(6): e1500251.
15 BLOODGOOD D W, SUGAM J A, HOLMES A, et al. Fear extinction requires infralimbic cortex projections to the basolateral amygdala[J]. Transl Psychiatry, 2018, 8(1): 60.
16 HUANG W C, ZUCCA A, LEVY J, et al. Social behavior is modulated by valence-encoding mPFC-amygdala sub-circuitry[J]. Cell Rep, 2020, 32(2): 107899.
17 FRANKLIN K B J, PAXINOS G. The mouse brain in stereotaxic coordinates [M]. 3rd ed. San Diego: Academic Press, 2008.
18 GERFEN C R, PALETZKI R, HEINTZ N. GENSAT BAC Cre-recombinase driver lines to study the functional organization of cerebral cortical and basal ganglia circuits[J]. Neuron, 2013, 80(6): 1368-1383.
19 MATHO K S, HUILGOL D, GALBAVY W, et al. Genetic dissection of the glutamatergic neuron system in cerebral cortex[J]. Nature, 2021, 598(7879): 182-187.
20 HE M, TUCCIARONE J, LEE S, et al. Strategies and tools for combinatorial targeting of GABAergic neurons in mouse cerebral cortex[J]. Neuron, 2016, 91(6): 1228-1243.
21 BOUWMEESTER H, SMITS K, VAN REE J M. Neonatal development of projections to the basolateral amygdala from prefrontal and thalamic structures in rat[J]. J Comp Neurol, 2002, 450(3): 241-255.
22 MCDONALD A J, MASCAGNI F, GUO L. Projections of the medial and lateral prefrontal cortices to the amygdala: a Phaseolus vulgaris leucoagglutinin study in the rat[J]. Neuroscience, 1996, 71(1): 55-75.
23 GABBOTT P L A, WARNER T A, JAYS P R L, et al. Prefrontal cortex in the rat: projections to subcortical autonomic, motor, and limbic centers[J]. J Comp Neurol, 2005, 492(2): 145-177.
24 HARRIS K D, SHEPHERD G M G. The neocortical circuit: themes and variations[J]. Nat Neurosci, 2015, 18(2): 170-181.
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