Journal of Shanghai Jiao Tong University (Medical Science) >

2022 , Vol. 42 >Issue 9: 1288 - 1295

DOI: https://doi.org/10.3969/j.issn.1674-8115.2022.09.015

Basic research

Screening of MUCIN family members synergistic with MUC1 in tumor chemoresistance

  • Kairan TANG ,
  • Qiong WU ,
  • Sijia HUANG ,
  • Xudong QIU ,
  • Wenyan LI ,
  • Huayun DENG ,
  • Lei HUANG
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  • Department of Histoembryology, Genetics and Developmental Biology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China
DENG Huayun, E-mail: denghuayun0796@shsmu.edu.cn.
HUANG Lei, E-mail: leihuang@shsmu.edu.cn

Received date: 2021-12-13

  Accepted date: 2022-03-09

  Online published: 2022-06-07

Supported by

National Natural Science Foundation of China(81874197);The 13th Innovation Training Program for Students of Shanghai Jiao Tong University School of Medicine(1319014);Experimental Technical Team Construction Project of Shanghai Municipal Education Commission(BJ1-3000-19-0135)

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Abstract

Objective ·To screen partners of mucin 1 (MUC1) in MUCIN family members that involve in chemoresistance. Methods ·Two pairs of cell lines were employed, including HeLa229/TR-CasCTL and HeLa229/TR-CasMUC1, and HeLa229/P and paclitaxel-resistant cell HeLa229/TR. Firstly, the cells were employed to detect the mRNA levels of MUCINs by quantitative real-time PCR (qPCR). The correlation of mRNA levels between MUC1 and MUCINs was analyzed. Then, Western blotting was performed to detect the protein levels of the selected MUCINs to get the proteins correlated with MUC1 at both mRNA and protein levels. IC50 assay was further employed to detect the role of target protein in paclitaxel resistance by knockdown of the gene through shRNA in HeLa229/TR cells. The mean expression levels of MUC1 and MUC13 in a variety of cancer tissues were further explored in the GEPIA database. The R2 database was further employed to detect the linear correlation of the expression between MUC1 and MUC13 in cervical, colon and pancreatic cancer tissues. Finally, the expression of MUC1 and MUC13 was analyzed by the Kaplan-Meier Plotter database in relation to the survival rate of patients with gastric cancer. Results ·The detection of qPCR showed that MUC13 and MUC18 exhibited correlation with MUC1 at the mRNA level in MUCIN family members. Western blotting showed that only MUC13 protein was up-regulated with the up-regulation of MUC1, while down-regulated with the silence of MUC1. IC50 assay displayed that silencing of MUC13 in HeLa229/TR decreased IC50 to paclitaxel. By searching the GEPIA database, the expression levels of MUC1 and MUC13 were simultaneous high or low in a variety of cancer tissues. From the R2 database, MUC1 and MUC13 showed a positive linear correlation in cervical, colon and pancreatic cancer tissues. In addition, the negative relationship of the expression of MUC1 and MUC13 and survival rate of patients with gastric cancer from the Kaplan-Meier Plotter database was observed. Conclusion ·MUC13 is a partner for MUC1 in promoting tumor chemoresistance. The expression levels of MUC1 and MUC13 are negatively related with the survival rate of cancer patients. It's feasible for joint inhibiting MUC1 and MUC13 to overcome chemoresistance.

Key words： MUCIN family; mucin 1 (MUC1); synergistic effect; chemoresistance

Cite this article

Kairan TANG , Qiong WU , Sijia HUANG , Xudong QIU , Wenyan LI , Huayun DENG , Lei HUANG . Screening of MUCIN family members synergistic with MUC1 in tumor chemoresistance[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2022 , 42(9) : 1288 -1295 . DOI: 10.3969/j.issn.1674-8115.2022.09.015

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