Journal of Shanghai Jiao Tong University (Medical Science) >

2023 , Vol. 43 >Issue 7: 814 - 820

DOI: https://doi.org/10.3969/j.issn.1674-8115.2023.07.003

Biomaterials and regenerative medicine column

Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta

  • Rujie ZHANG ,
  • Guangdong ZHOU ,
  • Jianbo WANG ,
  • Guangdong WENG ,
  • Dong LEI ,
  • Wenhui GONG
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  • 1.Department of Cardio-Vascular Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230088, China
    2.Department of Plastic and Reconstructive Surgery, Shanghai 9th People′s Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Lab of Tissue Engineering, Shanghai 200125, China
LEI Dong, E-mai: 370309803@qq.com. #Co-responding authors.
GONG Wenhui, E-mail: gongwenhui18@163.com

Received date: 2023-04-24

  Accepted date: 2023-07-17

  Online published: 2023-07-28

Supported by

Natural Science Foundation of Anhui Province(1908085MH241)

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Abstract

Objective ·To explore the feasibility of fluorinated decellularized rabbit aorta as a small artificial blood vessel for tissue engineering. Methods ·The obtained rabbit aorta was decellularized in combination with Triton X-100, sodium deoxycholate (SD), sodium dodecyl sulfate (SDS), DNAse, and RNAse. Hematoxylin-eosin staining (H-E staining), Masson staining and Verhoff-von Gieson staining were performed in the decellularized group and undecellularized group, respectively. The effect of decellularization was identified by field emission scanning electron microscope, and the morphological changes of decellularized blood vessels were observed. The decellularized rabbit aorta was used as the arterialized artificial small vessel scaffold, and the decellularized small vessel intima was modified with liquid perfluorocarbons coating to prepare a new type of artificial small vessel. The characteristic groups of the artificial small vessel were qualitatively and quantitatively determined. The dissipation time of liquid on the inner surface of the vessel and the flow of liquid on the surface of the vessel tilted at 45° were observed to analyze the hydrophobicity of the vessel. The blood vessels in the decellularized group and the fluorinated group were implanted with platelet-rich plasma, incubated, and observed under an electron microscope to evaluate the antiplatelet aggregation in vitro. The balloon pressure pump was connected to the aorta of the undecellularized group, decellularized group and fluoride group for bursting pressure test. Results ·Histological observation of blood vessels showed that the combination could effectively remove cells while retaining collagen and elastic fibers, and there was no damage to the intima under the electron microscope. There was no significant difference in the pressure blasting test among the three groups. In the hydrophobicity experiment, the retention time of water droplets on the membrane of the fluorinated group was over 5 min, and no obvious water marks were left on the 45° inclined plate. In the platelet adhesion test, intimal aggregation activated platelets in the decellularized group, while they were inhibited in the fluorinated group. Conclusion ·The decellularized blood vessels have good mechanical properties and physical stability by combined decellularization, and the fluorinated coating makes the blood vessels have good anticoagulant and biocompatibility.

Key words： decellularization; tissue engineering; rabbit; small blood vessel; intima; coating

Cite this article

Rujie ZHANG , Guangdong ZHOU , Jianbo WANG , Guangdong WENG , Dong LEI , Wenhui GONG . Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2023 , 43(7) : 814 -820 . DOI: 10.3969/j.issn.1674-8115.2023.07.003

References

1 LI J, LI X, WANG Q, et al. ST-segment elevation myocardial infarction in China from 2001 to 2011 (the China PEACE-Retrospective Acute Myocardial Infarction Study): a retrospective analysis of hospital data[J]. Lancet, 2015, 385(9966): 441-451.
2 YANG G, WANG Y, ZENG Y, et al. Rapid health transition in China, 1990-2010: findings from the Global Burden of Disease Study 2010[J]. Lancet, 2013, 381(9882): 1987-2015.
3 HAYWARD P A, GORDON I R, HARE D L, et al. Comparable patencies of the radial artery and right internal thoracic artery or saphenous vein beyond 5 years: results from the Radial Artery Patency and Clinical Outcomes trial[J]. J Thorac Cardiovasc Surg, 2010, 139(1): 60-67.
4 SUZUKI T, ASAI T, NOTA H, et al. Impact of total arterial reconstruction on long-term mortality and morbidity: off-pump total arterial reconstruction versus non-total arterial reconstruction[J]. Ann Thorac Surg, 2015, 100(6): 2244-2249.
5 BUXTON B F, SHI W Y, TATOULIS J, et al. Total arterial revascularization with internal thoracic and radial artery grafts in triple-vessel coronary artery disease is associated with improved survival[J]. J Thorac Cardiov Sur, 2014, 148(4): 1238-1244.
6 TARA S, ROCCO K A, HIBINO N, et al. Vessel bioengineering[J]. Circ J, 2014, 78(1): 12-19.
7 NEREM R M. Tissue engineering of the vascular system[J]. Vox Sang, 2004, 87(s2): 158-160.
8 LIAO J, XU B, ZHANG R H, et al. Applications of decellularized materials in tissue engineering: advantages, drawbacks and current improvements, and future perspectives[J]. J Mater Chem B, 2020, 8(44): 10023-10049.
9 DESMET W, VANHAECKE J, VROLIX M, et al. Isolated single coronary artery: a review of 50,000 consecutive coronary angiographies[J]. Eur Heart J, 1992, 13(12): 1637-1640.
10 MORONI F, MIRABELLA T. Decellularized matrices for cardiovascular tissue engineering[J]. Am J Stem Cells, 2014, 3(1): 1-20.
11 SWARTZ D D, ANDREADIS S T. Animal models for vascular tissue-engineering[J]. Curr Opin Biotechnol, 2013, 24(5): 916-925.
12 SONG L, DUAN P, ZHOU Q. Preparation and characterization of a de-cellularized rabbit aorta as a promising scaffold in vascular tissue engineering[J]. Cell Mol Biol (Noisy-le-grand), 2016, 62(3): 31-38.
13 JOUDA H, LARREA MURILLO L, WANG T. Current Progress in Vascular Engineering and Its Clinical Applications[J]. Cells, 2022, 11(3): 493.
14 JEONG Y, YAO Y, YIM E K F. Current understanding of intimal hyperplasia and effect of compliance in synthetic small diameter vascular grafts[J]. Biomater Sci, 2020, 8(16): 4383-4395.
15 STOWELL C E T, WANG Y. Quickening: translational design of resorbable synthetic vascular grafts[J]. Biomaterials, 2018, 173:71-86.
16 CHEN J P, SU C H. Surface modification of electro spun PLLA nanofibers by plasma treatment and cationized gelatin immobilization for cartilage tissue engineering[J]. Acta Biomater, 2011, 7(1): 234-243.
17 GAO M, LIANG C, SONG X J, et al. Erythrocyte-membrane-enveloped perfluorocarbon as nanoscale artificial red blood cells to relieve tumor hypoxia and enhance cancer radiotherapy[J]. Adv Mater, 2017, 29(35). DOI: 10.1002/adma.201701429.
18 FINK C, GAUDET J M, FOX M S, et al. 19F-perfluorocarbon-labeled human peripheral blood mononuclear cells can be detected in vivo using clinical MRI parameters in a therapeutic cell setting[J]. Sci Rep, 2018, 8(1): 590.
19 ROBERTS T R, HAREA G T, SINGHA P, et al. Heparin-free extracorporeal life support using tethered liquid perfluorocarbon: a feasibility and efficacy study[J]. ASAIO J, 2020, 66(7): 809-817.
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