Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta

doi:10.3969/j.issn.1674-8115.2023.07.003

Abstract

Abstract:

Objective ·To explore the feasibility of fluorinated decellularized rabbit aorta as a small artificial blood vessel for tissue engineering. Methods ·The obtained rabbit aorta was decellularized in combination with Triton X-100, sodium deoxycholate (SD), sodium dodecyl sulfate (SDS), DNAse, and RNAse. Hematoxylin-eosin staining (H-E staining), Masson staining and Verhoff-von Gieson staining were performed in the decellularized group and undecellularized group, respectively. The effect of decellularization was identified by field emission scanning electron microscope, and the morphological changes of decellularized blood vessels were observed. The decellularized rabbit aorta was used as the arterialized artificial small vessel scaffold, and the decellularized small vessel intima was modified with liquid perfluorocarbons coating to prepare a new type of artificial small vessel. The characteristic groups of the artificial small vessel were qualitatively and quantitatively determined. The dissipation time of liquid on the inner surface of the vessel and the flow of liquid on the surface of the vessel tilted at 45° were observed to analyze the hydrophobicity of the vessel. The blood vessels in the decellularized group and the fluorinated group were implanted with platelet-rich plasma, incubated, and observed under an electron microscope to evaluate the antiplatelet aggregation in vitro. The balloon pressure pump was connected to the aorta of the undecellularized group, decellularized group and fluoride group for bursting pressure test. Results ·Histological observation of blood vessels showed that the combination could effectively remove cells while retaining collagen and elastic fibers, and there was no damage to the intima under the electron microscope. There was no significant difference in the pressure blasting test among the three groups. In the hydrophobicity experiment, the retention time of water droplets on the membrane of the fluorinated group was over 5 min, and no obvious water marks were left on the 45° inclined plate. In the platelet adhesion test, intimal aggregation activated platelets in the decellularized group, while they were inhibited in the fluorinated group. Conclusion ·The decellularized blood vessels have good mechanical properties and physical stability by combined decellularization, and the fluorinated coating makes the blood vessels have good anticoagulant and biocompatibility.

Key words: decellularization, tissue engineering, rabbit, small blood vessel, intima, coating

CLC Number:

R654.3

ZHANG Rujie, ZHOU Guangdong, WANG Jianbo, WENG Guangdong, LEI Dong, GONG Wenhui. Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2023, 43(7): 814-820.

Figures/Tables 9

Fig 1 Acellular rabbit aorta

Fig 2 Pressure burst testing of the vessels

Fig 3 Histological observation of the decellularized group and non-decellularized group (×200)

Fig 4 FSEM scanning electron microscopy observation in the decellularized and undecellularized group

Fig 5 Surface morphology and elemental analysis of fluorine-containing coatings in blood vesselNote: A. SEM. B. EDS. cps—counts per second. eV—electron volt. 1 eV=1.602×10-19 J.

Tab 1 Elemental analysis of the intima of fluorinated small vessels

Element	Weight/%	Percentage of the atom/%
C	59.18	69.05
O	6.04	5.29
F	34.79	25.66
Total amount	100.00	100.00

Fig 6 Results of the vascular pressure burst test

Fig 7 Results of hydrophobicity of the intimal surface of the vessel

Fig 8 Results of the vascular antiplatelet adhesion test

TrendMD

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