Journal of Shanghai Jiao Tong University (Medical Science) >

2025 , Vol. 45 >Issue 1: 79 - 86

DOI: https://doi.org/10.3969/j.issn.1674-8115.2025.01.009

Techniques and methods

Optimization and evaluation of mouse model construction method for severe periodontitis

  • WANG Jiaxuan ,
  • ZHANG Qianqian ,
  • SUI Baiyan ,
  • LIU Xin
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  • Department of Dental Materials, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Institute of Stomatology, Shanghai 200011, China
LIU Xin, E-mail: liuxin8253@sjtu.edu.cn.

Received date: 2024-09-09

  Accepted date: 2024-09-27

  Online published: 2025-01-17

Supported by

National Natural Science Foundation of China(82271024);Cross Foundation of Ninth People′s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine(JYJC202309);Shanghai′s Top Priority Research Center(2022ZZ01017);Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2019-I2M-5-037)

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Abstract

Objective ·To investigate an optimal severe periodontitis mouse model by comparing two induction methods: simple ligature and ligature combined with injection of Porphyromonasgingivalis lipopolysaccharide (P.g. LPS). Methods ·Fifteen C57BL/6 mice were divided into three groups: a healthy control group, a simple ligature-induced periodontitis group, and a ligature combined with P.g. LPS injection-induced periodontitis group. After 14 d, the following evaluations were conducted: tooth mobility and probing depth under a stereomicroscope; alveolar bone resorption [bone volume fraction, bone mineral density, the distance from the cemento-enamel junction (CEJ) to the alveolar bone crest (ABC), and the area between CEJ and ABC] analyzed via micro computed tomography (Micro-CT) and stereomicroscopic examination. The serum levels of inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were assessed by enzyme-linked immunosorbent assay (ELISA). Results ·Compared with the simple ligature group, mice in the ligature with P.g. LPS injection group exhibited significantly increased tooth mobility [(2.20±0.45) vs (1.40±0.55)] and probing depth [(1.05±0.21) mm vs (0.58±0.39) mm], with statistically significant differences (P<0.05). The ligature with P.g. LPS injection group also demonstrated significantly reduced bone volume fraction [(16.44%±3.35%) vs (28.97%±7.90%)] and bone mineral density [(0.42±0.04) g/cm3vs (0.55±0.08) g/cm3], as well as increased distance from CEJ to ABC [(0.88±0.03) mm vs (0.74±0.12) mm] and area between CEJ and ABC [(0.34±0.01) mm2vs (0.30±0.02) mm2], all with statistically significant differences (all P<0.05). Additionally, serum levels of TNF-α and IL-1β were significantly elevated in the ligature with P.g. LPS injection group compared to the simple ligature group (both P<0.05). Conclusion ·The method of ligature combined with continuous P.g. LPS injection is more effective for constructing a severe periodontitis mouse model, making it suitable for studying the progression and treatment of severe periodontitis.

Key words： animal model; periodontitis; mouse; lipopolysaccharide (LPS)

Cite this article

WANG Jiaxuan , ZHANG Qianqian , SUI Baiyan , LIU Xin . Optimization and evaluation of mouse model construction method for severe periodontitis[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2025 , 45(1) : 79 -86 . DOI: 10.3969/j.issn.1674-8115.2025.01.009

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