Journal of Shanghai Jiao Tong University (Medical Science) >

2025 , Vol. 45 >Issue 7: 866 - 873

DOI: https://doi.org/10.3969/j.issn.1674-8115.2025.07.008

Basic research

Nanoengineered T cell system for the in vitro treatment of oral squamous cell carcinoma

  • MENG Jing ,
  • XIE Yuting ,
  • ZUO Jiaxin ,
  • XIONG Ping
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  • 1.Department of Ultrasound, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
    2.Department of Obstetrics and Gynecology, Fujian Maternal and Child Health Hospital affiliated to Fujian Medical University, Fuzhou 335001, China
XIONG Ping, E-mail: xiongpingxp@163.com.

Received date: 2025-01-08

  Accepted date: 2025-04-11

  Online published: 2025-07-28

Supported by

Project of Shanghai Science and Technology Commission(23ZR1438000);Project for Disciplinary Group Construction of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine(JYJC202132)

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Abstract

Objective ·To construct a nanoengineered T cell system and explore its synergistic efficacy in the in vitro treatment of oral squamous cell carcinoma, and provide a new strategy for this disease. Methods ·Zr-MOF nanoparticles with a highly hydrophilic hyaluronic acid (HA) coating (PCN224-HA NPs) were prepared, and their carboxyl groups were activated to enable coupling with anti-CD45 antibodies through an amide reaction. High-purity CD8+ T cells were then extracted from the spleens of mice, and the PCN224-HA NPs were loaded onto the T cells via the CD45 antigens on their surface. The coupling was observed using bioelectron microscopy and confocal microscopy, and free radical generation was detected under ultrasound irradiation. The viability of Cal27 cells was recorded by a microplate reader to explore therapeutic effects in vitro. Results ·PCN224-HA NPs were successfully synthesized and confirmed to be coupled onto the surface of CD8+ T cells, constructing a nanoengineered T cell system (T cells@PCN224-HA NPs). Free radical production was monitored after ultrasound irradiation and the viability of Cal27 cells decreased to 31.70% after in vitro treatment. Conclusions ·Preliminary experiments demonstrated that the nanoparticles can be successfully loaded onto the surface of T cells, providing combined sonodynamic therapeutic effects from the nanoparticles and immunocidal effects from the T cells. This offers a novel strategy for the treatment of oral squamous cell carcinoma, with potential for further in vivo anti-tumor experiments and therapeutic applications.

Key words： oral squamous cell carcinoma; sonodynamic therapy; immunotherapy; nanoengineered T cells; PCN224-HA nanoparticle; CD8+ T cell; synergistic therapy

Cite this article

MENG Jing , XIE Yuting , ZUO Jiaxin , XIONG Ping . Nanoengineered T cell system for the in vitro treatment of oral squamous cell carcinoma[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2025 , 45(7) : 866 -873 . DOI: 10.3969/j.issn.1674-8115.2025.07.008

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