Isolation and identification of cellulose-degrading bacteria under room temperature
Online published: 2010-08-27
Supported by
Foundation of Shanghai Jiaotong University School of Medicine, 2007XJ007;Shanghai Education Committee Foundation, 09ZZ111;Shanghai Science and Technology Committee Foundation, 09ZR1415500;Innovation Program for Undergraduates, 091024859
Objective To isolate and culture the bacteria that can degrade cellulose under room temperature. Methods Samples of decayed leaves (n=6), surface soil (n=6) and polluted water (n=2) were collected, and were seeded into multiple fiber media. Compound cellulose media were used for screening, carboxymethyl cellulose sodium(CMC-Na) plates were employed for isolation, enriched media were adopted for culture, and cellulose-degrading bacteria were obtained after cellulase activity detection under temperature of 22 ℃. Genomic DNA was extracted from these bacteria, and was sequenced with universal primers for 16S rDNA. The results of sequencing were aligned with the data of Genebank to identify the target bacteria. Results Three strains of cellulose-degrading bacteria were isolated, with the cellulase activity of 0.028 μg/min, 0.57 μg/min and 1.2 μg/min, respectively. Sequencing analysis revealed that the matching rates between these 3 strains of bacteria and Paenibacillus, Candidatus Chryseobacterium massiliae and Chryseobacterium were 99%, 97% and 97%, respectively. Conclusion Cellulose-degrading bacteria can be isolated from decayed leaves, surface soil and polluted water bodies under room temperature.
Key words: cellulose-degrading bacteria; isolation; screening; cellulose
CHEN Yan, ZHOU Sun-quan, ZHENG Qi-shi, et al . Isolation and identification of cellulose-degrading bacteria under room temperature[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2010 , 30(8) : 1018 . DOI: 10.3969/j.issn.1674-8115.2010.08.036
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