›› 2010, Vol. 30 ›› Issue (8): 1018-.doi: 10.3969/j.issn.1674-8115.2010.08.036

• Technique and method • Previous Articles    

Isolation and identification of cellulose-degrading bacteria under room temperature

CHEN Yan, ZHOU Sun-quan, ZHENG Qi-shi, ZHOU Yi-jun, WANG Yan, TAN You-ming   

  1. School of Public Health, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-08-25 Published:2010-08-27
  • Supported by:

    Foundation of Shanghai Jiaotong University School of Medicine, 2007XJ007；Shanghai Education Committee Foundation, 09ZZ111；Shanghai Science and Technology Committee Foundation, 09ZR1415500；Innovation Program for Undergraduates, 091024859

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Abstract:

Objective To isolate and culture the bacteria that can degrade cellulose under room temperature. Methods Samples of decayed leaves (n=6), surface soil (n=6) and polluted water (n=2) were collected, and were seeded into multiple fiber media. Compound cellulose media were used for screening, carboxymethyl cellulose sodium(CMC-Na) plates were employed for isolation, enriched media were adopted for culture, and cellulose-degrading bacteria were obtained after cellulase activity detection under temperature of 22 ℃. Genomic DNA was extracted from these bacteria, and was sequenced with universal primers for 16S rDNA. The results of sequencing were aligned with the data of Genebank to identify the target bacteria. Results Three strains of cellulose-degrading bacteria were isolated, with the cellulase activity of 0.028 μg/min, 0.57 μg/min and 1.2 μg/min, respectively. Sequencing analysis revealed that the matching rates between these 3 strains of bacteria and Paenibacillus, Candidatus Chryseobacterium massiliae and Chryseobacterium were 99%, 97% and 97%, respectively. Conclusion Cellulose-degrading bacteria can be isolated from decayed leaves, surface soil and polluted water bodies under room temperature.

Key words: cellulose-degrading bacteria, isolation, screening, cellulose