上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

p40同源二聚体对Th17细胞增殖及IL-17分泌的影响

薛 峰，李 霞，徐倩男，陈小英，郑 捷   

  1. 上海交通大学  医学院附属瑞金医院皮肤科， 上海 200025
  • 出版日期:2013-12-28 发布日期:2014-01-02
  • 通讯作者: 郑 捷, 电子信箱: jie-zheng2001@126.com。
  • 作者简介:薛 峰（1976—）， 男， 主管技师， 博士生; 电子信箱: xuefeng204108@ 126.com。
  • 基金资助:

    上海市卫生局基金（2011Y076）

Effects of p40 homodimer on T helper 17 cell proliferation and interleukin 17 secretion

XUE Feng, LI Xia, XU Qian-nan, CHEN Xiao-ying, ZHENG Jie   

  1. Department of  Dermatology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2013-12-28 Published:2014-01-02
  • Supported by:

    Foundation of  Shanghai Municipal Health Bureau, 2011Y076

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摘要：

目的 研究p40同源二聚体（p40）2对银屑病患者外周血辅助性T细胞亚群17 (Th17)功能的影响。方法 取C57BL/6小鼠骨髓，体外培养诱导分化Th0和Th17细胞，采用流式细胞术检测（p40）2对细胞增殖和细胞内白介素17 （IL-17）分泌的影响。提取正常人和银屑病患者外周血单个核细胞（PBMC），酶联免疫吸附ELISA法检测和分析（p40）2 对细胞培养上清中IL-17释放的影响。结果 流式细胞仪检测结果显示：加入(p40)2 对Th0细胞增殖无明显影响，而对Th17细胞增殖分裂有显著促进作用；与未加入（p40）2的Th17细胞比较，加入（p40）2 的Th17细胞能分泌更多的IL-17。与未加入(p40)2 的银屑病患者外周血PBMC比较，加入(p40)2 的银屑病患者外周血PBMC 培养上清中的IL-17含量显著增高（P<0.01），加入与未加入(p40)2的正常人PBMC培养上清中的IL-17含量比较差异无统计学意义（P>0.05）。结论 细胞因子（p40）2在银屑病发病中具有重要作用， 揭示了p40单抗治疗银屑病有效的可能机制。

关键词: p40同源二聚体, 辅助性T细胞亚群17, 银屑病

Abstract:

Objective To explore effects of p40 homodimer (p40) on T helper 17 cells in peripheral blood of patients with psoriasis. Methods Bone marrow cells of C57BL/6 mice were isolated and differentiated into bone marrow derived dendritic cells (BMDCs), and then BMDCs were cocultured with CD4+ T cells to differentiate in vitro into Th0 and Th17 cells. Fluorescence-activated cell sorting (FACS) was used to analyze IL-17 secretion and T cell proliferation in the presence or absence of  (p40)2. In addition, periphera blood mononuclear cells (PBMCs) from psoriasis patients and healthy donors were isolated and cultured with or without (p40)2. The IL-17 level in the culture supernatant was measured by ELISA. Results FACS analysis showed that (p40)2 had no obvious effect on Th0 cell proliferation whereas Th17 cell proliferation was significantly enhanced with high percentage of Th17 differentiation by adding (p40)2. In ELISA assay, the IL-17 concentration from cultures of PBMCs from psoriasis patients was significantly elevated upon adding (p40)2. In contrast, (p40)2 had little effect in augmenting the IL-17 level in healthy donors (P>0.05). Conclusion Cytokine (p40)2 plays a significant role in the pathogenesis of psoriasis by specifically promoting Th17 responses, which provides a possible explanation of the effectiveness of p40 monoclonal antibodies in treating psoriasis patients.

Key words: p40 homodimer, T helper 17 cell, psoriasis