上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

感染后肠易激综合征小鼠Cajal间质细胞改变对肠道动力和内脏敏感性的影响

杨 波1，蓝 程2，周旭春1   

  1. 1.重庆医科大学附属第一医院消化内科， 重庆 400016； 2.海南省人民医院消化内科， 海口 570311
  • 出版日期:2014-07-28 发布日期:2014-08-11
  • 通讯作者: 周旭春, 电子信箱: chqxchzh@163.com。
  • 作者简介:杨 波（1986—）， 男， 硕士生; 电子信箱: yblyl9527@126.com。
  • 基金资助:

    国家自然科学基金（81160057）

Effects of changes of interstitial cells of Cajal from mice with post-infection irritable bowel syndrome on intestinal motility and visceral sensitivity

YANG Bo1, LAN Cheng2, ZHOU Xu-chun1   

  1. 1.Department of Gastroenterology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China； 2.Department of Gastroenterology, Hainan Provincial People's Hospital, Haikou 570311, China
  • Online:2014-07-28 Published:2014-08-11
  • Supported by:

    National Natural Science Foundation of China, 81160057

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摘要：

目的 观察感染后肠易激综合征模型小鼠肠道Cajal间质细胞改变对肠道动力及内脏敏感性的影响。方法 34只雌性C57BL/6小鼠随机分为模型组和对照组，每组17只。模型组用0.2 mL含400条旋毛虫幼虫的生理盐水悬液灌胃，对照组给予等量生理盐水灌胃。于感染后第14、28和56日，取回肠末端和近端结肠组织进行病理学检查。感染后第56日，检测小鼠腹壁撤退反射（AWR）评分、肠道传输时间、每2 h的粪便粒数和Bristol评分；免疫组织化学染色和RT-PCR测定不同肠段Cajal间质细胞中c-kit蛋白和mRNA的表达。结果 感染后第56日，模型组小鼠肠道炎症完全消退；与对照组比较，模型组小鼠AWR评分显著增高（P<0.01），肠道传输时间明显缩短（P<0.01）；模型组小鼠每2 h的粪便粒数、湿重和Bristol评分均显著高于对照组（P<0.05）。免疫组织化学染色和RT-PCR检测结果显示：与对照组比较，模型组小鼠各肠段Cajal间质细胞中C-kit蛋白和mRNA表达均显著增强(P<0.05)。结论 感染后肠易激综合征小鼠肠道Cajal间质细胞改变可能是肠道动力紊乱和内脏敏感性增高的机制之一。

关键词: 感染后肠易激综合征, Cajal间质细胞, 肠道动力, 内脏敏感性

Abstract:

Objective To observe the effects of changes of interstitial cells of Cajal from mice with post-infection irritable bowel syndrome (PI-IBS) on the intestinal motility and visceral sensitivity. Methods Thirty four female C57BL/6 mice were randomly divided into the model group (n=17) and control group (n=17). Mice of the model group were intragastricly injected by normal saline of 0.2 mL with 400 larvae of trichina. Mice of the control group were intragastricly injected by normal saline of the same volume. Pathological examinations were conducted for tissues of terminal ileum and proximate colon 14, 28, and 56 d after infection. The abdominal withdrawal reflex (AWR) scores, intestine transportation time, fecal grain numbers for every 2 h, and Bristol scores were determined 56 d after infection. The expressions of c-kit protein and mRNA in interstitial cells of Cajal of different intestinal segments were detected by the immunohistochemical staining and RT-PCR. Results The intestinal inflammatory reaction of mice of the model group regressed completely 56 d after infection. Compared to the control group, the AWR scores of mice of the model group were significantly higher (P<0.01) and the intestine transportation time was significantly shorter (P<0.01). Grain numbers for every 2 h, wet weights, and Bristol scores of the model group were significantly higher than those of the control group (P<0.05). Results of the immunohistochemical staining and RTPCR showed that compared to the control group, the expressions of c-kit protein and mRNA in interstitial cells of Cajal of each intestinal segment of mice of the model group were significantly increased (P<0.05). Conclusion The changes of the interstitial cells of Cajal of mice with PI-IBS may be one of the mechanisms of intestinal motility disorders and visceral hypersensitivity.

Key words: post-infection irritable bowel syndrome, interstitial cells of Cajal, intestinal motility, visceral sensitivity