上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

p27kip1和Ki-67在原发性中枢神经系统生殖细胞肿瘤中表达的临床病理学意义

高玉平1，江基尧2，刘 强3   

  1. 上海交通大学医学院附属仁济医院 1.生殖医学中心， 上海市辅助生殖与优生重点实验室, 2.神经外科, 3.病理科, 上海 200127
  • 出版日期:2014-12-28 发布日期:2014-12-30
  • 作者简介:高玉平（1967—）， 女， 副主任医师， 博士; 电子信箱: ginagao53@yahoo.com。
  • 基金资助:

    上海市科委基金（134119a9502，12DZ2260600）

Clinicopathological significance of expressions of p27kip1 and Ki-67 in primary central nervous system germ cell tumors

GAO Yu-ping1, JIANG Ji-yao2, LIU Qiang3   

  1. 1.Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, 2.Department of Neurosurgery, 3.Department of Pathology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • Online:2014-12-28 Published:2014-12-30
  • Supported by:

    Foundation of Science and Technology Commission of Shanghai Municipality，134119a9502，12DZ2260600

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摘要：

目的 探讨抑癌基因p27kip1和细胞周期蛋白Ki-67在原发性中枢神经系统生殖细胞肿瘤(CNSGCTs)中的表达及其临床病理学意义。方法 采用免疫组织化学方法检测28例原发性CNSGCTs组织中p27kip1和Ki-67的表达，计算Ki-67增殖指数（Ki-67 LI）。结果 28例原发性CNSGCTs中，17例（60.7%）p27kip1高表达，其中成熟性畸胎瘤5例，生殖细胞瘤10例，胚胎性癌和混合性生殖细胞瘤各1例；11例（39.3%）p27kip1低表达，其中生殖细胞瘤8例，未成熟性畸胎瘤、混合性生殖细胞瘤、绒毛膜癌各1例。13例（46.4%）Ki-67LI>50%，9例（32.1%）Ki-67LI为25%～50%，6例（21.4%）Ki-67LI<25%。原发性CNSGCTs组织中p27kip1表达和Ki-67LI与肿瘤的组织学类型有关，p27kip1表达水平与患者性别、年龄，肿瘤大小、部位，预后和复发以及Ki-67LI均无相关性(P>0.05)。结论 p27kip1在恶性程度较高的原发性CNSGCTs中呈现低表达，提示其可能通过下调细胞周期G1→S的调控点而参与生殖细胞肿瘤的发生和发展。

关键词: 原发性中枢神经系统生殖细胞肿瘤, 生殖细胞瘤, 畸胎瘤, p27kip1, Ki-67

Abstract:

Objective To investigate the clinicopathological significance of expressions of tumor suppressor gene p27kip1 and expression of cyclin Ki-67 in primary central nervous system germ cell tumors (CNSGCTs). Methods The expressions of p27kip1 and Ki-67 in tissues of 28 cases of primary CNSGCTs were detected by immunohistochemical method and Ki-67 labeling index (Ki-67 LI) was calculated. Results Among 28 cases of primary CNSGCTs, the p27kip1 expression of 17 cases (60.7%) was high, including 5 cases of mature teratomas, 10 cases of germinomas, 1 case of embryonal carcinoma, and 1 case of mixed GCT; and the p27kip1 expression of 11 cases (39.3%) was low, including 8 cases of germinomas, 1 case of immature teratoma, 1 case of choriocarcinoma, and 1 case of mixed GCT. The Ki-67 LI of 13 cases (46.4％) was higher than 50%; Ki-67 LI of 9 cases (32.1%) was 25%-50%; and Ki-67 LI of 6 cases (21.4%) was lower than 25%. The expression of p27kip1 and Ki-67 LI correlated with the histological types of CNSGCTs. The expression of p27kip1 did not correlate with sex, age, size of tumor, location, recurrence, prognosis, or Ki-67 LI (P>0.05). Conclusion The expression of p27kip1 is low in malignant primary CNSGCTs, which suggests that p27kip1 may involve in the formation and development of germ cell tumor by down-regulating the control point for transiting from G1 to S phase.

Key words: primary central nervous system germ cell tumors, germ cell tumors, teratoma, p27kip1, Ki-67